Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety.
School of Food Science, Guangdong Pharmaceutical University.
J Oleo Sci. 2022;71(5):721-733. doi: 10.5650/jos.ess21353.
In this study, SBA-15 was functionalized by organic groups (-CH, -CH, -CH, -CHCHNH, -CH, et al.), and then Lecitase Ultra (LU) was immobilized onto the modified SBA-15 for soybean oil degumming. The hydrolysis activity, degumming performance, reusability in degumming, and the composition of phospholipids in the gum, of the immobilized LU samples, were carefully studied. Hydrolysis activities over 1800 U/g were obtained from all the immobilized LU samples. The highest activity of up to 4554.17 U/g was observed from the 3-ureidopropyl group-modified SBA-15-supported LU. Most of the immobilized LU samples removed the phospholipids effectively from crude soybean oil (initial phosphorous content 314.23 mg/kg), with a residual phosphorus content of less than 10 mg/kg. The reusability of the immobilized LU samples in the degumming process was evaluated. No loss of activity was observed from the methyl and N-(2-aminoethyl)-3-aminopropyl group-modified SBA-15-supported LU samples after five cycles of reuse. In addition, 3-aminopropyl and 3-glycidyloxypropyl group-modified SBA-15-supported LU samples retained over 90% of their initial activity; N-phenylaminomethyl and 1-isocyanatopropane group-functionalized SBA-15-supported LU samples retained approximately 80% of their initial activity. The phospholipids in the gum were analyzed. The n-octadecyl and N-(2-aminoethyl)-3-aminopropyl group-functionalized SBA-15-supported LU samples were selective for lysophosphatidylethanolamine (LPE) preparation, and LPE percentages up to 37.14 and 38.80% were obtained, respectively. The N-phenylaminomethyl group-modified SBA-15-supported LU showed selectivity toward lysophosphatidylcholine (LPC) production, with an LPC percentage of up to 38.5%.
在这项研究中，SBA-15 通过有机基团（-CH、-CH、-CH、-CHCHNH、-CH 等）进行功能化，然后将 Lecitase Ultra（LU）固定在改性 SBA-15 上用于大豆油脱胶。仔细研究了固定化 LU 样品的水解活性、脱胶性能、在脱胶中的可重复使用性以及胶中磷脂的组成。所有固定化 LU 样品的水解活性均超过 1800 U/g。从 3-脲丙基改性 SBA-15 负载的 LU 观察到高达 4554.17 U/g 的最高活性。大多数固定化 LU 样品有效地从粗大豆油中去除了磷脂（初始磷含量 314.23mg/kg），残留磷含量低于 10mg/kg。评估了固定化 LU 样品在脱胶过程中的可重复使用性。经过五次重复使用后，甲基和 N-(2-氨基乙基)-3-氨基丙基改性 SBA-15 负载的 LU 样品没有失去活性。此外，3-氨基丙基和 3-缩水甘油氧基丙基改性 SBA-15 负载的 LU 样品保留了超过 90%的初始活性；N-苯甲胺甲基和 1-异氰酸丙基改性 SBA-15 负载的 LU 样品保留了大约 80%的初始活性。对胶中的磷脂进行了分析。正十八烷基和 N-(2-氨基乙基)-3-氨基丙基改性 SBA-15 负载的 LU 样品对制备溶血磷脂酰乙醇胺（LPE）具有选择性，分别获得高达 37.14%和 38.80%的 LPE 百分比。N-苯甲胺甲基改性 SBA-15 负载的 LU 对溶血磷脂酰胆碱（LPC）的生成具有选择性，最高可达 38.5%的 LPC 百分比。
