Guangdong Provincial Key Laboratory of Applied Botany & Agriculture and Biotechnology Research Center, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
University of Chinese Academy of Sciences, Beijing, China.
J Cell Physiol. 2022 Jul;237(7):2961-2968. doi: 10.1002/jcp.30761. Epub 2022 May 1.
Histone deacetylases (HDACs) play important roles in the repression of gene expression. Our previous study revealed that HISTONE DEACETYLASE 9 (HDA9) interacts with ELONGATED HYPOCOTYL 5 (HY5) and is involved in regulating plant autophagy in response to the light-to-dark transition and nitrogen starvation. In this study, we observed that the hda9-1 and hy5-215 single mutants flowered earlier compared with the wild-type Col-0; in addition, the hda9-1 hy5-215 double mutant flowered earlier than each single mutant. The expression of several positive flowering time genes was upregulated in the hda9-1, hy5-215, and hda9-1 hy5-215 mutants. Chromatin immunoprecipitation analysis demonstrated that HDA9 and HY5 bound directly to the promoter regions of PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) and CONSTANS-LIKE 5 (COL5) and repressed their expression through H3K9 and H3K27 deacetylation. Taken together, our results reveal the epigenetic mechanism explaining how the HDA9-HY5 module functions in controlling flowering time.
组蛋白脱乙酰酶 (HDACs) 在基因表达的抑制中发挥重要作用。我们之前的研究表明，HISTONE DEACETYLASE 9 (HDA9) 与 ELONGATED HYPOCOTYL 5 (HY5) 相互作用，参与调节植物自噬以响应光暗转换和氮饥饿。在这项研究中，我们观察到 hda9-1 和 hy5-215 单突变体比野生型 Col-0 开花更早；此外，hda9-1 hy5-215 双突变体比每个单突变体开花更早。几个正向开花时间基因的表达在 hda9-1、hy5-215 和 hda9-1 hy5-215 突变体中上调。染色质免疫沉淀分析表明，HDA9 和 HY5 直接结合到 PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) 和 CONSTANS-LIKE 5 (COL5) 的启动子区域，并通过 H3K9 和 H3K27 脱乙酰化来抑制它们的表达。总之，我们的结果揭示了组蛋白脱乙酰酶 9-ELONGATED HYPOCOTYL 5 模块通过表观遗传机制控制开花时间的机制。
