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核心技术专利：CN118964589B侵权必究
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一种低成本生物打印机改装的设计与实现，可实现塑料挤出与凝胶挤出之间的切换。

Design and implementation of a low cost bio-printer modification, allowing for switching between plastic and gel extrusion.

作者信息

Krige Adolf, Haluška Jakub, Rova Ulrika, Christakopoulos Paul

机构信息

Biochemical Process Engineering, Division of Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, SE-971 87 Luleå, Sweden.

出版信息

HardwareX. 2021 Feb 26;9:e00186. doi: 10.1016/j.ohx.2021.e00186. eCollection 2021 Apr.

DOI:10.1016/j.ohx.2021.e00186
PMID:35492054
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9041258/
Abstract

Due to the high cost of bioprinters they are not feasible for proof of concept experiments or educational purposes. Furthermore, the more affordable DIY methods all disable the plastic printing capability of the original printer. Here we present an affordable bio-printing modification that is easy to install and maintains the original capabilities of the printer. The modification used mostly 3D printed parts and is based on the popular, open-source Prusa i3 3D printer. The modifications are kept as simple as possible and uses standard slicing software, allowing for installation by less experienced builders. By using disposable syringes and easily sterilizable parts, an aseptic bioprinting setup can be achieved, depending on the environment. It also allows for 2 component printing as well as UV curing. The bio-printing and curing capabilities were shown by printing and curing an artificial biofilm of an electro-active bacteria, Geobacter sulfurreducens, onto a carbon-cloth electrode which was used in a microbial fuel cell.

摘要

由于生物打印机成本高昂，它们对于概念验证实验或教育目的而言并不可行。此外，更经济实惠的DIY方法都会禁用原始打印机的塑料打印功能。在此，我们展示一种经济实惠的生物打印改装方法，该方法易于安装且能保持打印机的原始功能。这种改装主要使用3D打印部件，并且基于广受欢迎的开源Prusa i3 3D打印机。改装尽可能保持简单，并使用标准切片软件，使经验较少的组装人员也能进行安装。通过使用一次性注射器和易于消毒的部件，根据环境情况可实现无菌生物打印设置。它还支持双组分打印以及紫外线固化。通过将电活性细菌——硫还原地杆菌的人工生物膜打印并固化到用于微生物燃料电池的碳布电极上，展示了生物打印和固化能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/4bd355c1f561/gr15.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/81d9d835fff1/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/2ceeb5e7b474/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/912d56b8d80f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/45a7b9ed40d6/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/ad8704f9a243/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/5c59c986e5b9/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/c118d9156e35/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/29fca85c90e7/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/1d3f813683ea/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/0df36edf0131/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/c1e711f2e8ad/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/de36565f8f88/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/9d48eb13d0e2/gr12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/3088f748ab13/gr13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/b5111d496814/gr14.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/4bd355c1f561/gr15.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/81d9d835fff1/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/2ceeb5e7b474/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/912d56b8d80f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/45a7b9ed40d6/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/ad8704f9a243/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/5c59c986e5b9/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/c118d9156e35/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/29fca85c90e7/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/1d3f813683ea/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/0df36edf0131/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/c1e711f2e8ad/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/de36565f8f88/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/9d48eb13d0e2/gr12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/3088f748ab13/gr13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/b5111d496814/gr14.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/98b1/9041258/4bd355c1f561/gr15.jpg

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本文引用的文献

[1]
Ultra-Low-Cost 3D Bioprinting: Modification and Application of an Off-the-Shelf Desktop 3D-Printer for Biofabrication.

Front Bioeng Biotechnol. 2019-7-31

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