Lehner Benjamin A E, Schmieden Dominik T, Meyer Anne S
Department of Bionanoscience, TU Delft , 2628 CD Delft, Netherlands.
ACS Synth Biol. 2017 Jul 21;6(7):1124-1130. doi: 10.1021/acssynbio.6b00395. Epub 2017 Mar 1.
Sustainable and personally tailored materials production is an emerging challenge to society. Living organisms can produce and pattern an extraordinarily wide range of different molecules in a sustainable way. These natural systems offer an abundant source of inspiration for the development of new environmentally friendly materials production techniques. In this paper, we describe the first steps toward the 3-dimensional printing of bacterial cultures for materials production and patterning. This methodology combines the capability of bacteria to form new materials with the reproducibility and tailored approach of 3D printing systems. For this purpose, a commercial 3D printer was modified for bacterial systems, and new alginate-based bioink chemistry was developed. Printing temperature, printhead speed, and bioink extrusion rate were all adapted and customized to maximize bacterial health and spatial resolution of printed structures. Our combination of 3D printing technology with biological systems enables a sustainable approach for the production of numerous new materials.
可持续且个性化定制的材料生产是社会面临的一项新挑战。生物体能够以可持续的方式生产并塑造种类极为繁多的不同分子。这些自然系统为开发新型环保材料生产技术提供了丰富的灵感来源。在本文中，我们描述了用于材料生产和图案化的细菌培养物三维打印的初步步骤。这种方法将细菌形成新材料的能力与三维打印系统的可重复性和定制方法相结合。为此，对一台商用三维打印机进行了改造以适用于细菌系统，并开发了基于藻酸盐的新型生物墨水化学配方。对打印温度、打印头速度和生物墨水挤出速率进行了调整和定制，以最大限度地提高细菌的健康状况和打印结构的空间分辨率。我们将三维打印技术与生物系统相结合，为生产众多新材料提供了一种可持续方法。
