Frighetto Giovanni, Zordan Mauro A, Castiello Umberto, Megighian Aram, Martin Jean-René
Department of General Psychology, University of Padova, Padova, Italy.
Institut des Neurosciences Paris-Saclay, Université Paris-Saclay, CNRS, Saclay, France.
Front Physiol. 2022 Apr 14;13:849142. doi: 10.3389/fphys.2022.849142. eCollection 2022.
The central complex (CX) is a neural structure located on the midline of the insect brain that has been widely studied in the last few years. Its role in navigation and goal-oriented behaviors resembles those played by the basal ganglia in mammals. However, the neural mechanisms and the neurotransmitters involved in these processes remain unclear. Here, we exploited an bioluminescence Ca imaging technique to record the activity in targeted neurons of the ellipsoid body (EB). We used different drugs to evoke excitatory Ca-responses, depending on the putative neurotransmitter released by their presynaptic inputs, while concomitant dopamine administration was employed to modulate those excitations. By using a genetic approach to knockdown the dopamine 1-like receptors, we showed that different dopamine modulatory effects are likely due to specific receptors expressed by the targeted population of neurons. Altogether, these results provide new data concerning how dopamine modulates and shapes the response of the ellipsoid body neurons. Moreover, they provide important insights regarding the similitude with mammals as far as the role played by dopamine in increasing and stabilizing the response of goal-related information.
中央复合体（CX）是位于昆虫脑中线的一种神经结构，在过去几年中受到了广泛研究。它在导航和目标导向行为中的作用类似于哺乳动物基底神经节所起的作用。然而，这些过程中涉及的神经机制和神经递质仍不清楚。在这里，我们利用一种生物发光钙成像技术来记录椭球体（EB）中目标神经元的活动。我们使用不同的药物来诱发兴奋性钙反应，这取决于它们突触前输入释放的假定神经递质，同时给予多巴胺来调节这些兴奋。通过使用基因方法敲低多巴胺1样受体，我们表明不同的多巴胺调节作用可能是由于目标神经元群体表达的特定受体所致。总之，这些结果提供了关于多巴胺如何调节和塑造椭球体神经元反应的新数据。此外，它们还提供了关于多巴胺在增加和稳定目标相关信息反应中所起作用方面与哺乳动物相似性的重要见解。
