Sun Xiao-Ya, Liu Teng, Sun Jie, Wang Xiao-Jing
School of Medicine and Life Sciences, University of Jinan, Shandong Academy of Medical Sciences Jinan 250200 Shandong China
Key Laboratory for Biotech-Drugs Ministry of Health Jinan 250062 Shandong China.
RSC Adv. 2020 Mar 17;10(18):10826-10847. doi: 10.1039/c9ra10290f. eCollection 2020 Mar 11.
In recent years, the research on fluorescent probes has developed rapidly. Coumarin fluorescent probes have also been one of the hot topics in recent years. For the synthesis and application of coumarin fluorescent probes, great progress has been made. Coumarin fluorescent probes have become more and more widely used in biochemistry, environmental protection, and disease prevention, and have broad prospects. This review introduces the three main light emitting mechanisms (PET, ICT, FRET) of fluorescent probes, and enumerates some probes based on this light emitting mechanism. In terms of the synthesis of coumarin fluorescent probes, the existing substituents on the core of coumarin compounds were modified. Based on the positions of the modified substituents, some of the fluorescent probes reported in the past ten years are listed. Most of the fluorescent probes are formed by modifying the 3 and 7 position substituents on the mother nucleus, and the 4 and 8 position substituents are relatively less modified. In terms of probe applications, the detection and application of coumarin fluorescent probes for Cu, Hg, Mg, Zn, pH, environmental polarity, and active oxygen and sulfide in the past ten years are mainly introduced.
近年来，荧光探针的研究发展迅速。香豆素荧光探针也是近年来的热点话题之一。在香豆素荧光探针的合成与应用方面，已经取得了很大进展。香豆素荧光探针在生物化学、环境保护和疾病预防等领域的应用越来越广泛，具有广阔的前景。本文综述了荧光探针的三种主要发光机制（PET、ICT、FRET），并列举了基于这种发光机制的一些探针。在香豆素荧光探针的合成方面，对香豆素化合物核心上现有的取代基进行了修饰。根据修饰取代基的位置，列出了过去十年报道的一些荧光探针。大多数荧光探针是通过修饰母核上的3位和7位取代基形成的，而4位和8位取代基的修饰相对较少。在探针应用方面，主要介绍了过去十年香豆素荧光探针在检测和应用铜、汞、镁、锌、pH值、环境极性以及活性氧和硫化物等方面的情况。
