Attia Mohamed S, Ahmed Amal M, Amin Tarek A, Youssef Ahmed O, Amin Mohammed A, Mohamed Ekram H, Mahmoud Safwat A, Abou-Omar Mona N
Chemistry Department, Faculty of Science, Ain Shams University Cairo 11566 Egypt
Department of Chemistry, Collage of Science, Taif University P. O. BOX 11099 Taif 21944 Saudi Arabia.
RSC Adv. 2021 Oct 6;11(52):32861-32872. doi: 10.1039/d1ra05368j. eCollection 2021 Oct 4.
An innovative, simple and cost effective Tb-simvastatin photo probe was designed and used as a core for a spectrofluorometric approach to sensitively determine four vital biological compounds in different matrices. A Tb-simvastatin complex displays a characteristic electrical band with at 545 nm with significant luminescence intensity, which is quenched in the presence of folic acid, progesterone, testosterone and vitamin D at four variant sets of pH: 5.0, 6.2, 7.5 and 9.0, respectively. The conditions were optimized and the best solvent for operation was found to be acetonitrile at at 340 nm. Folic acid was successfully estimated in tablet dosage form, urine and serum in the concentration range of 2.49 × 10 to 1.28 × 10 mol L. Progesterone, testosterone and vitamin D were also assessed in serum samples using the same optimal conditions within concentration ranges of 5 × 10 to 1.9 × 10, 5 × 10 to 2.8 × 10 and 5 × 10 to 4.2 × 10 mol L, respectively. The proposed luminescence method was validated in accordance with ICH guidelines and found to be accurate, precise, and specific and free from any interference at the working pH for each analyte. The cost effectiveness and applicability of the method make it a good choice for routine analysis of the four compounds and early diagnosis of chronic diseases associated with abnormalities in their physiological levels.
设计了一种创新、简单且经济高效的Tb-辛伐他汀光探针，并将其用作荧光光谱法的核心，以灵敏地测定不同基质中的四种重要生物化合物。Tb-辛伐他汀络合物在545 nm处显示出特征性的电波段，具有显著的发光强度，在叶酸、孕酮、睾酮和维生素D存在下，分别在pH值为5.0、6.2、7.5和9.0的四个不同组中，该发光强度会猝灭。对条件进行了优化，发现最佳的操作溶剂是乙腈，激发波长为340 nm。成功测定了片剂剂型、尿液和血清中浓度范围为2.49×10至1.28×10 mol/L的叶酸。在相同的最佳条件下，还对血清样品中的孕酮、睾酮和维生素D进行了评估，其浓度范围分别为5×10至1.9×10、5×10至2.8×10和5×10至4.2×10 mol/L。所提出的发光方法按照ICH指南进行了验证，结果表明该方法准确、精密、特异，并且在每种分析物的工作pH值下无任何干扰。该方法的成本效益和适用性使其成为这四种化合物常规分析以及与其生理水平异常相关的慢性疾病早期诊断的良好选择。
