Du Bairen, Zhang Jingjing, Dong Yanjie, Wang Junwei, Lei Longwen, Shi Rengfei
College of Sport, Anqing Normal University, Anqing 246011, Anhui, China.
School of Kinesiology, Shanghai University of Sport, Shanghai 200438, China.
Steroids. 2020 Sep;161:108691. doi: 10.1016/j.steroids.2020.108691. Epub 2020 Jun 27.
A novel method for determining the testosterone/epitestosterone concentration ratio in human urine was established by capillary electrophoresis with diode-array detector. The urine samples were firstly purified by the solid extraction. The optimal experimental conditions were: running buffer pH = 4.74, 15.0 mmol L HAc-NaAc, separation voltage 25 kV, temperature 25 °C, sample injection pressure 3.43 × 10 Pa, and duration 10 s. The testosterone and epitestosterone linear range were determined as 8.0-960.0 ng mL, respectively. The testosterone and epitestosterone detection limits were determined as 4.6 and 4.5 ng mL, respectively. The relative standard deviation was less than 0.36%.
建立了一种采用二极管阵列检测器的毛细管电泳法测定人尿中睾酮/表睾酮浓度比的新方法。尿样首先通过固相萃取进行纯化。最佳实验条件为：运行缓冲液pH = 4.74，15.0 mmol L HAc-NaAc，分离电压25 kV，温度25°C，进样压力3.43×10 Pa，持续时间10 s。睾酮和表睾酮的线性范围分别确定为8.0 - 960.0 ng mL。睾酮和表睾酮的检测限分别确定为4.6和4.5 ng mL。相对标准偏差小于0.36%。
