Anhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Sciences, Hefei, China.
Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Front Cell Infect Microbiol. 2022 Apr 14;12:823820. doi: 10.3389/fcimb.2022.823820. eCollection 2022.
Fowl adenovirus serotype 4 (FAdV-4) caused hepatitis-hydropericardium syndrome in poultry and caused huge economic losses to the poultry industry. At present, antiviral drugs have not been reported to be effective against this virus, and new treatment methods are urgently needed to treat FAdV-4. Camptothecin has been shown to have antiviral activity against various viruses; however, whether it can inhibit FAdV-4 infection remains unclear. This study aimed to explore the anti-FAdV-4 effects and mechanisms of camptothecin and . Several camptothecin treatments were used to study the antiviral activity of camptothecin on FAdV-4-infected Leghorn male hepatocellular (LMH) cells. The FAdV-4 titers of mock and camptothecin-treated infected cell cultures were determined using tissue culture infective dose assay, and the FAdV-4 copy number was determined using quantitative real-time polymerase chain reaction. In addition, the therapeutic effect of camptothecin on FAdV-4-infected chickens was also evaluated. The results showed that camptothecin significantly reduced the viral replication in LMH cells in a dose-dependent manner, resulting in a reduction in viral titer, viral copy number, and viral Hexon protein expression. Camptothecin was also found to have a significant inhibitory effect on the viral replication step. Finally, camptothecin showed anti-FAdV-4 efficacy in the chicken infection model, and the survival rate was improved. This study was novel in proving that camptothecin had a protective effect against FAdV-4, indicating its potential as an antiviral drug against FAdV-4 infection.
禽腺病毒血清型 4（FAdV-4）可引起家禽的肝炎-心包积水综合征，给家禽业造成了巨大的经济损失。目前，尚未有报道称抗病毒药物对此种病毒有效，因此急需寻找新的治疗方法来治疗 FAdV-4。喜树碱已被证明对多种病毒具有抗病毒活性；然而，其是否能抑制 FAdV-4 感染尚不清楚。本研究旨在探索喜树碱及其衍生物抑制禽腺病毒 4 型（FAdV-4）的作用机制。通过使用几种喜树碱处理来研究喜树碱对感染 FAdV-4 的莱航雄性鸡肝细胞（LMH）细胞的抗病毒活性。通过组织培养感染剂量测定法测定 mock 和喜树碱处理感染细胞培养物中的 FAdV-4 滴度，通过定量实时聚合酶链反应测定 FAdV-4 拷贝数。此外，还评估了喜树碱对感染 FAdV-4 的鸡的治疗效果。结果表明，喜树碱可显著降低 LMH 细胞中的病毒复制，呈剂量依赖性，导致病毒滴度、病毒拷贝数和病毒 Hexon 蛋白表达降低。喜树碱还被发现对病毒复制步骤具有显著的抑制作用。最后，喜树碱在鸡感染模型中显示出抗 FAdV-4 的功效，提高了存活率。本研究的新颖之处在于证明了喜树碱对 FAdV-4 具有保护作用，表明其具有作为抗 FAdV-4 感染的抗病毒药物的潜力。
