Wang Dongwei, Xiao Dongqin, Lu Mengjie, Liu Qing, Xie Tao, Feng Gang, Weng Jie, Duan Ke
Collaborative Innovation Center of Tissue Repair Materials of Sichuan Province, College of Life Sciences, China West Normal University Nanchong Sichuan 637009 China
Research Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital, The Second Clinical College of North Sichuan Medical College Sichuan 637000 China.
RSC Adv. 2020 Feb 18;10(12):7251-7258. doi: 10.1039/c9ra08246h. eCollection 2020 Feb 13.
Bone implants are susceptible to postoperative infections. Immobilization of antibiotic-loaded microparticles on implants is an effective approach to addressing this problem. Immobilization methods reported in earlier studies frequently used special or potentially harmful conditions. Therefore, the present study explored a new method to immobilize poly(lactide--glycolide) (PLGA) microspheres on bone implant materials. PLGA microspheres were prepared by an emulsion method using polyvinyl alcohol (PVA) as an emulsifier. The microspheres were immobilized on two commonly used orthopaedic biomaterials [hydroxyapatite-coated titanium (HA-Ti) and poly(methyl methacrylate) (PMMA)] by dispersing on the surface followed by vacuum drying. Microspheres were retained stably on both materials even after immersion in phosphate-buffered saline for 12 d. Pretreatment of microspheres with sodium borate (, an eliminator of hydroxyl groups of PVA) substantially reduced their retention on HA-Ti, but only moderately reduced their retention on PMMA. This suggested that the binding of the residual PVA on the microspheres to the HA coating is the dominant contributor to their immobilization on HA-Ti, whereas other forces contributed substantially to their immobilization on PMMA. Microspheres containing ciprofloxacin (a water-soluble antibiotic) and triclosan (an oil-soluble antibiotic) were immobilized on HA-Ti and PMMA, respectively. They effectively killed adjacent bacteria. These results offer a simple and versatile method for immobilizing drug-release microspheres on some important bone implant surfaces.
骨植入物易发生术后感染。将负载抗生素的微粒固定在植入物上是解决这一问题的有效方法。早期研究报道的固定方法经常使用特殊或潜在有害的条件。因此，本研究探索了一种将聚（丙交酯-乙交酯）（PLGA）微球固定在骨植入材料上的新方法。PLGA微球采用以聚乙烯醇（PVA）为乳化剂的乳液法制备。通过分散在表面然后真空干燥，将微球固定在两种常用的骨科生物材料[羟基磷灰石涂层钛（HA-Ti）和聚甲基丙烯酸甲酯（PMMA）]上。即使在磷酸盐缓冲盐水中浸泡12天后，微球仍稳定地保留在两种材料上。用硼酸钠（PVA羟基的消除剂）对微球进行预处理，显著降低了它们在HA-Ti上的保留率，但仅适度降低了它们在PMMA上的保留率。这表明微球上残留的PVA与HA涂层的结合是它们固定在HA-Ti上的主要因素，而其他作用力对它们固定在PMMA上有很大贡献。分别将含有环丙沙星（一种水溶性抗生素）和三氯生（一种油溶性抗生素）的微球固定在HA-Ti和PMMA上。它们有效地杀死了邻近的细菌。这些结果提供了一种简单且通用的方法，可将药物释放微球固定在一些重要的骨植入物表面。
