Alamar Blue 在细胞活力生物测定中作为代谢功能和细胞健康指示剂的多种应用。
Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays.
机构信息
Department of Life Sciences, The University of the West Indies, West Indies, St Augustine, Trinidad and Tobago.
出版信息
Sensors (Basel). 2012;12(9):12347-60. doi: 10.3390/s120912347. Epub 2012 Sep 10.
Abstract
Accurate prediction of the adverse effects of test compounds on living systems, detection of toxic thresholds, and expansion of experimental data sets to include multiple toxicity end-point analysis are required for any robust screening regime. Alamar Blue is an important redox indicator that is used to evaluate metabolic function and cellular health. The Alamar Blue bioassay has been utilized over the past 50 years to assess cell viability and cytotoxicity in a range of biological and environmental systems and in a number of cell types including bacteria, yeast, fungi, protozoa and cultured mammalian and piscine cells. It offers several advantages over other metabolic indicators and other cytotoxicity assays. However, as with any bioassay, suitability must be determined for each application and cell model. This review seeks to highlight many of the important considerations involved in assay use and design in addition to the potential pitfalls.
摘要
准确预测测试化合物对生命系统的不良影响、检测毒性阈值以及扩大实验数据集以包括多种毒性终点分析，这些都是任何稳健筛选方案所必需的。Alamar Blue 是一种重要的氧化还原指示剂，用于评估代谢功能和细胞健康。在过去的 50 年中，Alamar Blue 生物测定法已被用于评估一系列生物和环境系统以及多种细胞类型（包括细菌、酵母、真菌、原生动物以及培养的哺乳动物和鱼类细胞）中的细胞活力和细胞毒性。它与其他代谢指示剂和其他细胞毒性测定法相比具有几个优点。但是，与任何生物测定法一样，必须为每种应用和细胞模型确定适用性。本综述旨在除了潜在的陷阱之外，还强调了测定使用和设计中涉及的许多重要考虑因素。
相似文献
1
Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays.Alamar Blue 在细胞活力生物测定中作为代谢功能和细胞健康指示剂的多种应用。
Sensors (Basel). 2012;12(9):12347-60. doi: 10.3390/s120912347. Epub 2012 Sep 10.
2
High-content image analysis (HCIA) assay has the highest correlation with direct counting cell suspension compared to the ATP, WST-8 and Alamar blue assays for measurement of cytotoxicity.与用于细胞毒性测量的ATP、WST-8和alamar蓝检测法相比，高内涵图像分析（HCIA）检测法与直接计数细胞悬液的相关性最高。
J Pharmacol Toxicol Methods. 2017 Nov;88(Pt 1):92-99. doi: 10.1016/j.vascn.2017.08.003. Epub 2017 Aug 12.
3
Resazurin Live Cell Assay: Setup and Fine-Tuning for Reliable Cytotoxicity Results.刃天青活细胞检测：为获得可靠的细胞毒性结果进行设置与微调
Methods Mol Biol. 2017;1647:207-219. doi: 10.1007/978-1-4939-7201-2_14.
4
A new, simple, nonradioactive, nontoxic in vitro assay to monitor corneal endothelial cell viability.一种用于监测角膜内皮细胞活力的新型、简单、非放射性、无毒的体外检测方法。
Invest Ophthalmol Vis Sci. 1997 Sep;38(10):1929-33.
5
Determination of Metabolic Viability and Cell Mass Using a Tandem Resazurin/Sulforhodamine B Assay.使用刃天青/磺酰罗丹明B串联检测法测定代谢活力和细胞量
Curr Protoc Toxicol. 2016 May 4;68:2.24.1-2.24.15. doi: 10.1002/cptx.1.
6
Basic Colorimetric Proliferation Assays: MTT, WST, and Resazurin.基本比色法增殖检测：MTT、WST和刃天青。
Methods Mol Biol. 2017;1601:1-17. doi: 10.1007/978-1-4939-6960-9_1.
7
Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity.用于评估哺乳动物细胞细胞毒性的阿拉玛蓝（刃天青）荧光染料的研究。
Eur J Biochem. 2000 Sep;267(17):5421-6. doi: 10.1046/j.1432-1327.2000.01606.x.
8
Assessment of a simple, non-toxic Alamar blue cell survival assay to monitor tomato cell viability.评估一种简单、无毒的阿拉玛蓝细胞存活测定法以监测番茄细胞活力。
Phytochem Anal. 2001 Sep-Oct;12(5):340-6. doi: 10.1002/pca.595.
9
Complex Formation of Resorufin and Resazurin with Β-Cyclodextrins: Can Cyclodextrins Interfere with a Resazurin Cell Viability Assay?Resorufin 和 Resazurin 与 β-环糊精的络合作用：环糊精会干扰 Resazurin 细胞活力测定吗？
Molecules. 2018 Feb 10;23(2):382. doi: 10.3390/molecules23020382.
10
Caveats to the use of MTT, neutral red, Hoechst and Resazurin to measure silver nanoparticle cytotoxicity.MTT、中性红、Hoechst 和 Resazurin 用于测量银纳米颗粒细胞毒性的注意事项。
Chem Biol Interact. 2020 Jan 5;315:108868. doi: 10.1016/j.cbi.2019.108868. Epub 2019 Oct 24.
引用本文的文献
1
Comparative Analysis of Polyphenol-Rich Extracts from Leaves and Bark: ROS Scavenging and Anti-Inflammatory Effects on Skin Cells.树叶和树皮中富含多酚提取物的比较分析：对皮肤细胞的活性氧清除及抗炎作用
Molecules. 2025 Aug 31;30(17):3572. doi: 10.3390/molecules30173572.
2
Behavior of Osteoblastic Lineage Cells When in the Presence of Tamoxifen: In Vitro and In Vivo Studies on Osseointegration.成骨细胞系细胞在他莫昔芬存在时的行为：骨整合的体外和体内研究
Dent J (Basel). 2025 Aug 1;13(8):351. doi: 10.3390/dj13080351.
3
Mechanism of bactericidal efficacy against nosocomial pathogenic Staphylococcus aureus strain caused by fatty acids from Hermetia illucens larvae fat.黑水虻幼虫脂肪中脂肪酸对医院致病性金黄色葡萄球菌菌株的杀菌作用机制
Sci Rep. 2025 Aug 19;15(1):30305. doi: 10.1038/s41598-025-15858-0.
4
Development of fatty acid analogues with potent anabolic effects on bone in male mice.对雄性小鼠骨骼具有强效合成代谢作用的脂肪酸类似物的研发。
Bone Rep. 2025 Jul 30;26:101862. doi: 10.1016/j.bonr.2025.101862. eCollection 2025 Sep.
5
Nutrient restriction protects against valve interstitial cell calcification by upregulating ubiquitin mediated proteolysis.营养限制通过上调泛素介导的蛋白水解作用来预防瓣膜间质细胞钙化。
Front Cardiovasc Med. 2025 Jul 21;12:1586775. doi: 10.3389/fcvm.2025.1586775. eCollection 2025.
6
Lactobacilli-based postbiotic differentially affects chicken macrophage-like HD11 cells depending on stimulatory lipopolysaccharide dosage.基于乳酸杆菌的后生元对鸡巨噬细胞样HD11细胞的影响因刺激脂多糖剂量而异。
BMC Vet Res. 2025 Jul 21;21(1):479. doi: 10.1186/s12917-025-04902-w.
7
Piezoelectricity of hexagonal boron nitrides improves bone tissue generation as tested on osteoblasts.在成骨细胞上进行测试时，六方氮化硼的压电性可促进骨组织生成。
Beilstein J Nanotechnol. 2025 Jul 7;16:1068-1081. doi: 10.3762/bjnano.16.78. eCollection 2025.
8
Evaluating Biocompatibility: From Classical Techniques to State-of-the-Art Functional Proteomics.评估生物相容性：从经典技术到最先进的功能蛋白质组学
Nanomaterials (Basel). 2025 Jul 3;15(13):1032. doi: 10.3390/nano15131032.
9
Intercellular communication between extracellular vesicles from conditioned macrophages and breast cancer cells drives endocrine therapy resistance.条件巨噬细胞来源的细胞外囊泡与乳腺癌细胞之间的细胞间通讯导致内分泌治疗耐药。
Front Cell Dev Biol. 2025 Jun 3;13:1548724. doi: 10.3389/fcell.2025.1548724. eCollection 2025.
10
Comparative Analysis of PRV-1 in Atlantic Salmon and PRV-3 in Coho Salmon: Host-Specific Immune Responses and Apoptosis in Red Blood Cells.大西洋鲑鱼中PRV-1与银大麻哈鱼中PRV-3的比较分析：宿主特异性免疫反应及红细胞凋亡
Microorganisms. 2025 May 21;13(5):1167. doi: 10.3390/microorganisms13051167.
本文引用的文献
1
A Rapid Method to Quantify Fungicide Sensitivity in the Brown Rot Pathogen Monilinia fructicola.一种快速量化褐腐病菌（核果链核盘菌）对杀菌剂敏感性的方法。
Plant Dis. 2009 Apr;93(4):328-331. doi: 10.1094/PDIS-93-4-0328.
2
A Rapid Colorimetric Microtiter Bioassay to Evaluate Fungicide Sensitivity Among Verticillium dahliae Isolates.一种用于评估大丽轮枝菌分离株中杀菌剂敏感性的快速比色微量滴定生物测定法。
Plant Dis. 2011 Mar;95(3):248-255. doi: 10.1094/PDIS-10-10-0725.
3
Differential Responses of Colletotrichum gloeosporioides and C. truncatum Isolates from Different Hosts to Multiple Fungicides Based on Two Assays.基于两种测定方法，来自不同寄主的胶孢炭疽菌和截形炭疽菌分离株对多种杀菌剂的差异反应
Plant Dis. 2012 Oct;96(10):1526-1536. doi: 10.1094/PDIS-10-11-0906-RE.
4
A Rapid Resazurin-Based Microtiter Assay to Evaluate QoI Sensitivity for Alternaria alternata Isolates and Their Molecular Characterization.一种基于刃天青的快速微量滴定法，用于评估链格孢菌分离株对甲氧基丙烯酸酯类杀菌剂的敏感性及其分子特征分析
Plant Dis. 2012 Sep;96(9):1262-1270. doi: 10.1094/PDIS-12-11-1037-RE.
5
The use of fish-derived cell lines for investigation of environmental contaminants.利用鱼类来源的细胞系研究环境污染物。
Curr Protoc Toxicol. 2003;Chapter 1:Unit 1.5. doi: 10.1002/0471140856.tx0105s15.
6
Casein kinase 2 inhibition attenuates androgen receptor function and cell proliferation in prostate cancer cells.酪蛋白激酶 2 抑制可减弱前列腺癌细胞中雄激素受体的功能和细胞增殖。
Prostate. 2012 Sep 15;72(13):1423-30. doi: 10.1002/pros.22493. Epub 2012 Jan 30.
7
Toward toxicity testing of nanomaterials in the 21st century: a paradigm for moving forward.迈向 21 世纪的纳米材料毒性测试：前进的范例。
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2012 Jan-Feb;4(1):1-15. doi: 10.1002/wnan.162. Epub 2011 Sep 30.
8
A new fluorometric assay for cytotoxicity measurements in-vitro.一种用于体外细胞毒性测量的新型荧光测定法。
Int J Oncol. 1993 Sep;3(3):473-6.
9
Pulmonary fibroblasts from COPD patients show an impaired response of elastin synthesis to TGF-β1.COPD 患者的肺成纤维细胞对 TGF-β1 的弹性蛋白合成反应受损。
Respir Physiol Neurobiol. 2011 Aug 15;177(3):236-40. doi: 10.1016/j.resp.2011.04.019. Epub 2011 Apr 22.
10
Cell viability assays: introduction.细胞活力测定：引言。
Methods Mol Biol. 2011;740:1-6. doi: 10.1007/978-1-61779-108-6_1.
Zotero 插件