Niaz Taskeen, Shabbir Saima, Noor Tayyaba, Imran Muhammad
Department of Biosciences, Faculty of Sciences, COMSATS University Islamabad, Park Road, Islamabad, Pakistan.
Pakistan Institute of Rehabilitation Sciences (PIRS), ISRA University Islamabad Campus, Islamabad, Pakistan.
Food Bioproc Tech. 2022;15(6):1284-1298. doi: 10.1007/s11947-022-02815-2. Epub 2022 Apr 23.
To meet the demands for more effective and ecofriendly food packaging strategies, the potential of nisin-loaded rhamnolipid functionalized nanofillers (rhamnosomes) has been explored after embedding in hydroxypropyl-methylcellulose (HPMC) and κ-carrageenan (κ-CR)-based packaging films. It was observed that intrinsically active rhamnosomes based nanofillers greatly improved the mechanical and optical properties of nano-active packaging (NAP) films. Incorporation of rhamnosomes resulted in higher tensile strength (5.16 ± 0.06 MPa), Young's modulus (2777 ± 0.77 MPa), and elongation (2.58 ± 0.03%) for NAP than active packaging containing free nisin (2.96 ± 0.03 MPa, 1107 ± 0.67 MPa, 1.48 ± 0.06%, respectively). NAP demonstrated a homogenous distribution of nanofillers in the biopolymer matrix as elucidated by scanning electron microscopy (SEM). Thermogravimetric analysis (TGA) confirmed that NAP prepared with nisin-loaded rhamnosomes was thermally stable even above 200 °C. Differential scanning calorimetry (DSC) analyses revealed that addition of nisin in nanofillers resulted in a slight increase in Tg (108.40 °C), indicating thermal stability of NAP. Fourier transform infrared spectroscopy (FTIR) revealed slight shift in all characteristic bands of nano-active packaging, which indicated the embedding of rhamnosomes inside the polymer network without any chemical interaction. Finally, when tested on chicken breast filets and cheese slices under refrigerated storage conditions, NAP demonstrated broad-spectrum antimicrobial activity (up to 4.5 log unit reduction) and inhibited the growth of , , , and . These results suggest that HPMC and κ-CR-based NAP containing functionalized nanofillers can serve as an innovative packaging material for the food industry to improve the safety, quality, and shelf-life of dairy and meat products.
The online version contains supplementary material available at 10.1007/s11947-022-02815-2.
为满足对更有效和环保型食品包装策略的需求，在将载有乳链菌肽的鼠李糖脂功能化纳米填料（鼠李糖体）嵌入羟丙基甲基纤维素（HPMC）和κ-卡拉胶（κ-CR）基包装薄膜后，对其潜力进行了探索。据观察，基于鼠李糖体的具有内在活性的纳米填料极大地改善了纳米活性包装（NAP）薄膜的机械和光学性能。与含有游离乳链菌肽的活性包装（分别为2.96±0.03 MPa、1107±0.67 MPa、1.48±0.06%）相比，加入鼠李糖体使NAP的拉伸强度更高（5.16±0.06 MPa）、杨氏模量更高（2777±0.77 MPa）以及伸长率更高（2.58±0.03%）。扫描电子显微镜（SEM）表明，NAP在生物聚合物基质中纳米填料分布均匀。热重分析（TGA）证实，用载有乳链菌肽的鼠李糖体制备的NAP即使在200℃以上仍具有热稳定性。差示扫描量热法（DSC）分析表明，在纳米填料中添加乳链菌肽会使玻璃化转变温度（Tg）略有升高（108.40℃），这表明NAP具有热稳定性。傅里叶变换红外光谱（FTIR）显示纳米活性包装的所有特征谱带都有轻微位移，这表明鼠李糖体嵌入了聚合物网络中且没有任何化学相互作用。最后，在冷藏储存条件下对鸡胸肉片和奶酪片进行测试时，NAP表现出广谱抗菌活性（减少高达4.5个对数单位），并抑制了 、 、 和 的生长。这些结果表明，含有功能化纳米填料的基于HPMC和κ-CR的NAP可作为食品工业的创新包装材料，以提高乳制品和肉类产品的安全性、质量和保质期。
在线版本包含可在10.1007/s11947-022-02815-2获取的补充材料。
