Aoki Kazusa, Osako Ryuji, Deng Jiahui, Hayashita Takashi, Hashimoto Takeshi, Suzuki Yumiko
Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University Kioi-cho 7-1, Chiyoda-ku Tokyo 102-8554 Japan
RSC Adv. 2020 Apr 17;10(26):15299-15306. doi: 10.1039/d0ra01455a. eCollection 2020 Apr 16.
Detection and visualization of phosphates such as ATP in living organisms can facilitate the elucidation of various biological events. Although substantial efforts had been made in this area, present methods have disadvantages such as the need for specialized equipment and poor sensitivities. To address these limitations, novel fluorescent probes, (di-(2-picolyl)amino)quinazolines, were developed for application in ATP detection. They selectively recognized copper ions by fluorescence quenching, and their copper complexes displayed fluorescence enhancement in the presence of phosphoric acid derivatives. This fluorescence on-off system enabled highly sensitive fluorescence detection of ATP when combined with a phenyl boronic acid-modified γ-cyclodextrin through a plausible multipoint recognition system.
检测和可视化生物体内的磷酸盐（如ATP）有助于阐明各种生物事件。尽管在这一领域已经做出了大量努力，但目前的方法存在一些缺点，如需要专门设备且灵敏度较低。为了解决这些局限性，开发了新型荧光探针（二（2-吡啶甲基）氨基喹唑啉）用于ATP检测。它们通过荧光猝灭选择性识别铜离子，并且它们的铜配合物在磷酸衍生物存在下显示荧光增强。当通过合理的多点识别系统与苯基硼酸修饰的γ-环糊精结合时，这种荧光开关系统能够对ATP进行高灵敏度荧光检测。
