Identification of Antibacterial Activity of From Antarctic Notothenioid Fish.

is a small peptide composed of signal peptide, propeptide, and the bioactive mature peptide from N terminal to C terminal. Mature is an antibacterial peptide and iron regulator with eight highly conserved cysteines forming four intramolecular disulfide bonds, giving it a β sheet hairpin-like structure. homologs are found in a variety of vertebrates, especially fish, and their diversity may be associated with different habitats and different levels of pathogens. , an Antarctic notothenioid fish that lives in the coldest water unlike most places of the world, with at least two variants with eight cysteines. We confirmed the formation process of activated mature from in Chinese hamster ovary (CHO) cell line, obtained recombinant protein from prokaryotes, and characterized its binding ability and antibacterial activity against varying bacteria. The expression of in CHO cell line showed that the prepropeptide of and cleavage into smaller mature peptide. The antibacterial assay and flow cytometry showed that , , and bound to different bacteria and killed them with different minimum inhibitory concentration. These data suggest that plays an important role in the innate immunity of and is of great value in improving resistance to pathogens.