State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, PR China.
State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, PR China; Section of Infection and Immunity, Herman Ostrow School of Dentistry of USC, Los Angeles, 90089, USA.
Fish Shellfish Immunol. 2020 Mar;98:551-563. doi: 10.1016/j.fsi.2020.01.038. Epub 2020 Jan 22.
Hepcidin, a multifunctional hormone oligopeptide, not only exhibits a regulatory role in iron metabolism, but also participates in the regulation of teleostean immunity. In this study, ORF sequence of WR-hepcidin was 258 bp and encoded 85 amino acid residues. Tissue-specific analysis revealed that the highest expression of WR-hepcidin was observed in liver. Aeromonas hydrophila challenge can sharply increased WR-hepcidin mRNA expression in liver, trunk kidney and spleen. The purified WR-hepcidin fusion peptide can directly bind to A. hydrophila and Streptococcus agalactiae, reduce the relative bacterial activity, limit bacterial growth and attenuate their dissemination to tissues in vivo. In addition, the treatment of WR-hepcidin fusion protein can diminish the production of pro-inflammatory cytokines. These results indicated that WR-hepcidin can play a negative regulatory role in bacteria-stimulated pro-inflammatory cytokines production and MyD88-IRAK4 activation.
亚铁调素（Hepcidin）是一种多功能激素寡肽，不仅在铁代谢中具有调节作用，还参与了硬骨鱼类免疫的调节。本研究中，WR-hepcidin 的 ORF 序列为 258bp，编码 85 个氨基酸残基。组织特异性分析显示，WR-hepcidin 在肝脏中的表达最高。哈维氏弧菌刺激可显著增加肝脏、中肾和脾脏中 WR-hepcidin mRNA 的表达。纯化的 WR-hepcidin 融合肽可直接与哈维氏弧菌和无乳链球菌结合，降低相对细菌活性，限制细菌生长，并减弱其在体内向组织传播。此外，WR-hepcidin 融合蛋白的处理可以减少促炎细胞因子的产生。这些结果表明，WR-hepcidin 可以在细菌刺激的促炎细胞因子产生和 MyD88-IRAK4 激活中发挥负调节作用。
