Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA.
Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA.
STAR Protoc. 2022 Apr 19;3(2):101334. doi: 10.1016/j.xpro.2022.101334. eCollection 2022 Jun 17.
This protocol describes how inductively coupled plasma mass spectrometry (ICP-MS) can quantify metals, sulfur, and phosphorus present in biological specimens. The high sensitivity of ICP-MS enables detection of these elements at very low concentrations, and absolute quantification is achieved with standard curves. Sulfur or phosphorus standardization reduces variability that arises because of slight differences in sample composition. This protocol bypasses challenges because of limited sample amounts and facilitates studies examining the biological roles of metals in health and disease. For complete details on the use and execution of this protocol, please refer to Hartwig et al. (2020).
本方案描述了如何使用电感耦合等离子体质谱法（ICP-MS）定量分析生物样本中的金属、硫和磷。ICP-MS 的高灵敏度可实现对这些元素的痕量检测，并通过标准曲线进行绝对定量。使用硫或磷标准品可减少因样品组成略有差异而导致的变异性。本方案可解决因样品量有限而产生的问题，并有助于研究金属在健康和疾病中的生物学作用。有关本方案的详细使用和实施信息，请参考 Hartwig 等人（2020 年）。
