一种优化的基于 FusX 组装的技术，用于在人细胞系中引入线粒体 TC-to-TT 变异。
An optimized FusX assembly-based technique to introduce mitochondrial TC-to-TT variations in human cell lines.
机构信息
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA.
Mayo Clinic Graduate School of Biomedical Sciences, Virology and Gene Therapy Track, Mayo Clinic, Rochester, MN, USA.
出版信息
STAR Protoc. 2022 Apr 14;3(2):101288. doi: 10.1016/j.xpro.2022.101288. eCollection 2022 Jun 17.
The FusX TALE Based Editor (FusXTBE) is a programmable base editing platform that can introduce specific TC-to-TT variations in the mitochondrial DNA (mtDNA). Here, we provide a protocol describing the synthesis and testing of the FusXTBE plasmids in cultured human cell lines. This tool is designed to be easily modified to work in diverse applications where editing of mitochondrial DNA is desired. For complete details on the use and execution of this protocol, please refer to Sabharwal et al. (2021) and Ma et al. (2016).
FusX TALE 基编辑工具（FusXTBE）是一个可编程的碱基编辑平台，可在线粒体 DNA（mtDNA）中引入特定的 TC 到 TT 变异。在这里，我们提供了一个描述 FusXTBE 质粒在培养的人细胞系中合成和测试的方案。该工具旨在易于修改，以应用于需要编辑线粒体 DNA 的各种应用中。有关此方案的使用和执行的完整详细信息，请参考 Sabharwal 等人（2021 年）和 Ma 等人（2016 年）的研究。
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