使用 MitoPunch 线粒体转移技术在哺乳动物细胞中生成稳定的分离线粒体受体克隆。
Generating stable isolated mitochondrial recipient clones in mammalian cells using MitoPunch mitochondrial transfer.
机构信息
Molecular Biology Interdepartmental Doctoral Program, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
出版信息
STAR Protoc. 2021 Sep 28;2(4):100850. doi: 10.1016/j.xpro.2021.100850. eCollection 2021 Dec 17.
This protocol describes the assembly and use of MitoPunch to deliver mitochondria containing mitochondrial DNA (mtDNA) into cells lacking mtDNA (ρ0 cells). MitoPunch generates stable isolated mitochondrial recipient clones with restored mtDNA and recovered respiration, enabling investigation of mtDNA mutations and mtDNA-nuclear DNA interactions in a range of cell types. For complete details on the use and execution of this protocol, please refer to Sercel et al. (2021) and Patananan et al. (2020).
本方案描述了使用 MitoPunch 将含有线粒体 DNA（mtDNA）的线粒体递送到缺乏 mtDNA（ρ0 细胞）的细胞中的方法。MitoPunch 可生成具有恢复呼吸功能的稳定的分离线粒体受体克隆，从而能够在多种细胞类型中研究 mtDNA 突变和 mtDNA-核 DNA 相互作用。如需了解本方案使用和执行的完整详细信息，请参考 Sercel 等人（2021 年）和 Patananan 等人（2020 年）的文献。
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