构建具有大角度共振光栅扫描的高 NFOM 多光子显微镜。

Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning.

机构信息

Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan.

Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan.

出版信息

STAR Protoc. 2022 Apr 22;3(2):101330. doi: 10.1016/j.xpro.2022.101330. eCollection 2022 Jun 17.

DOI:10.1016/j.xpro.2022.101330

PMID:35496804

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9048148/

Abstract

A resonant-scanning multiphoton optical microscope (MPM) with a millimeter-scale field-of-view (FOV) often encounters a poor Nyquist figure-of-merit (NFOM), leading to an aliasing effect owing to limited effective voxel-sampling rate. In this protocol, we provide a design guideline to enable high-NFOM MPM imaging while simultaneously securing a large FOV/digital-resolution ratio and a fast resonant raster-scanning speed. We further provide a free version of our custom acquisition software to assist with a smooth and easy construction process. For complete details on the use and execution of this protocol, please refer to Borah et al. (2021).

摘要

一种具有毫米级视场（FOV）的共振扫描多光子显微镜（MPM）通常会遇到较差的奈奎斯特品质因数（NFOM），这是由于有效体素采样率有限而导致的混叠效应。在本方案中，我们提供了一种设计指南，可实现高 NFOM MPM 成像，同时确保大 FOV/数字分辨率比和快速共振光栅扫描速度。我们还提供了我们自定义采集软件的免费版本，以协助进行顺畅和轻松的构建过程。有关本方案使用和执行的完整详细信息，请参阅 Borah 等人（2021 年）。