Li Jiawei, Xu Ouwen, Zhu Xiashi
School of Chemistry & Chemical Engineering, College of Guangling, Yangzhou University Yangzhou 225002 PR China
RSC Adv. 2021 Oct 20;11(54):34107-34116. doi: 10.1039/d1ra05656e. eCollection 2021 Oct 18.
In this study, an on-off-on fluorescence probe for the detection of trace Cu(ii) and ascorbic acid (AA) based on biomass-derived sulfur and nitrogen double heteroatom-doped carbon dots (N,S-CDs) was designed. For the first time, the probe (N,S-CDs) was prepared from grape seeds and thiourea as the precursor. Cu(ii) was added to the carbon point solution, the fluorescence intensity (FL) of N,S-CDs was strongly quenched (switch OFF) and the fluorescence probe turned to "ON" (switch ON) with the addition of AA. Under the optimal conditions, the as-synthesized N,S-CDs had a good detection performance for Cu(ii) and AA assay with the linearity ranges from 150-500 μg mL and 0.1-400 μg mL, and the LODs were 0.048 mg L and 0.036 mg L, respectively. The as-prepared N,S-CDs exhibited a low cytotoxicity and a good biocompatibility, which show their potential for application in the biological imaging of living cells.
在本研究中，设计了一种基于生物质衍生的硫和氮双杂原子掺杂碳点（N，S-CDs）的用于检测痕量铜（II）和抗坏血酸（AA）的开关型荧光探针。首次以葡萄籽和硫脲为前驱体制备了该探针（N，S-CDs）。向碳点溶液中加入铜（II），N，S-CDs的荧光强度（FL）被强烈猝灭（关闭开关），加入AA后荧光探针变为“开启”（打开开关）。在最佳条件下，合成的N，S-CDs对铜（II）和AA检测具有良好的性能，线性范围分别为150 - 500 μg/mL和0.1 - 400 μg/mL，检测限分别为0.048 mg/L和0.036 mg/L。所制备的N，S-CDs表现出低细胞毒性和良好的生物相容性，这表明它们在活细胞生物成像中的应用潜力。
