Wang Liyong, Wang Zhenghao, Wang Zhiwen, Zhang Chunyang, Wu Yongling, Zheng Hongyu
Centre for Advanced Laser Manufacturing (CALM), School of Mechanical Engineering, Shandong University of Technology Zibo 255000 Shandong China
College of Life Sciences, Shandong University of Technology Zibo 255000 Shandong China.
RSC Adv. 2021 Oct 18;11(54):33883-33889. doi: 10.1039/d1ra06390a.
Bacterial biofilms formed on touchable surfaces such as displays of electronic devices not only reduce the product service life, but also cause human health issues. There is an urgent need to research the biofilm formation mechanism and methodologies to prevent formation of biofilms on human touchable surfaces. It has been reported that laser-induced graphene (LIG) helps resist biofilm growth, which has been attributed to the atomic composition and sharp edges of graphene. However, LIG alone was not able to retard bacterial growth completely. It has been reported that LIG incorporated with silver (Ag) nanoparticles exhibited enhanced surface antibacterial activity. As a heavy metal, overdose of Ag is harmful to human health. Therefore, a new biocompatible antibacterial agent to replace or reduce the use of Ag is highly important. In this study, we investigate and compare the effect of LIG doped with two types of nanocrystals, , ZnO and silver (Ag)-doped ZnO, on antibacterial actions. A 355 nm ultraviolet (UV) laser was used to produce LIG on a watercolor paper substrate. Formation of few-layer graphene has been verified by Raman spectra. (), a representative of Gram-negative bacteria and (), a representative of Gram-positive bacteria were employed for the investigation of the bacteriostatic properties of the LIG paper substrate. Results show that with the incorporation of either the ZnO nanocrystals or the silver (Ag)-doped ZnO nanocrystals into LIG, the antibacterial effect became stronger. It is further shown that the Ag-doped ZnO nanocrystals have superior antibacterial performance to that of the ZnO nanocrystals. The Ag-doped ZnO nanocrystals are potentially an effective and biocompatible antibacterial agent and yet have a much reduced and acceptable level of Ag concentration.
在诸如电子设备显示屏等可触摸表面形成的细菌生物膜不仅会缩短产品使用寿命，还会引发人类健康问题。迫切需要研究生物膜形成机制以及防止在人类可触摸表面形成生物膜的方法。据报道，激光诱导石墨烯（LIG）有助于抵抗生物膜生长，这归因于石墨烯的原子组成和尖锐边缘。然而，仅LIG并不能完全抑制细菌生长。据报道，与银（Ag）纳米颗粒结合的LIG表现出增强的表面抗菌活性。作为一种重金属，过量的银对人类健康有害。因此，一种新型的生物相容性抗菌剂来替代或减少银的使用非常重要。在本研究中，我们研究并比较了掺杂两种纳米晶体（氧化锌（ZnO）和银（Ag）掺杂的ZnO）的LIG对抗菌作用的影响。使用355nm紫外（UV）激光在水彩纸基材上制备LIG。通过拉曼光谱验证了少层石墨烯的形成。使用革兰氏阴性菌代表大肠杆菌（Escherichia coli）和革兰氏阳性菌代表金黄色葡萄球菌（Staphylococcus aureus）来研究LIG纸基材的抑菌性能。结果表明，将ZnO纳米晶体或银（Ag）掺杂的ZnO纳米晶体掺入LIG中，抗菌效果会增强。进一步表明，Ag掺杂的ZnO纳米晶体具有比ZnO纳米晶体更优异的抗菌性能。Ag掺杂的ZnO纳米晶体可能是一种有效且生物相容的抗菌剂，并且银浓度水平大大降低且可接受。
