Coni Pierpaolo, Piras Monica, Mateddu Anna, Piludu Marco, Orru Germano, Scano Alessandra, Cabras Tiziana, Piras Valentina, Lachowicz Joanna Izabela, Jaremko Mariusz, Faa Gavino, Castagnola Massimo, Pichiri Giuseppina
Department of Medical Sciences and Public Health, University of Cagliari Cagliari Italy
Department of Biomedical Sciences, University of Cagliari Cagliari Italy.
RSC Adv. 2020 Mar 30;10(21):12680-12688. doi: 10.1039/c9ra10365a. eCollection 2020 Mar 24.
Biomarkers of cell stress are important for proper diagnosis, and in studies of how cells respond to drug treatment. Biomarkers that respond early to pharmacological treatment could improve therapy by tailoring the treatment to the needs of the patient. Thymosin beta-4 (Tβ) plays a significant role in many aspects of cellular metabolism because of its actin-sequestering properties. Other physiological functions of Tβ have been also reported. Among these, Tβ may play a crucial role during cellular stress. We addressed the relevance of Tβ in cellular stress conditions by using different treatments (serum starvation, DMSO, and butyrate administration) in a colon adenocarcinoma cell line (CaCo2), a cell line frequently used for experimental studies of Tβ. In this study, different stress stimuli were analyzed and the obtained results were compared using immunocytochemistry, and molecular and biochemical methods. Taken together, the data clearly indicate that the Tβ peptide is involved in adaptive and defensive cellular mechanisms, and that different stress inducers lead to a similar Tβ cytoplasmic/nuclear translocation. The translocation of Tβ between the cytoplasm and the nucleus of the cell seems characteristic of a possible molecular response to cellular stress exerted by this peptide.
细胞应激生物标志物对于正确诊断以及细胞如何响应药物治疗的研究非常重要。对药物治疗早期做出反应的生物标志物可以根据患者的需求调整治疗方案，从而改善治疗效果。胸腺素β-4（Tβ）因其肌动蛋白隔离特性在细胞代谢的许多方面发挥着重要作用。Tβ的其他生理功能也有报道。其中，Tβ可能在细胞应激期间发挥关键作用。我们通过在结肠腺癌细胞系（CaCo2）中使用不同的处理方法（血清饥饿、二甲基亚砜和丁酸盐给药）来探讨Tβ在细胞应激条件下的相关性，CaCo2是常用于Tβ实验研究的细胞系。在本研究中，分析了不同的应激刺激，并使用免疫细胞化学以及分子和生化方法对所得结果进行了比较。综合来看，数据清楚地表明Tβ肽参与了细胞的适应性和防御机制，并且不同的应激诱导剂会导致类似的Tβ细胞质/核易位。Tβ在细胞的细胞质和细胞核之间的易位似乎是该肽对细胞应激可能产生的分子反应的特征。
