Lau Soon Kiat, Subbiah Jeyamkondan
Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE, USA.
Department of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA.
HardwareX. 2020 Jun 7;8:e00114. doi: 10.1016/j.ohx.2020.e00114. eCollection 2020 Oct.
The determination of the thermal death kinetics of microorganisms has traditionally been performed with liquid baths which have some disadvantages such as liquid spillage and liquid infiltration into samples. The TDT Sandwich was developed as a free, open source alternative that utilizes dry heat. The system is capable of heating samples up to 140 °C and maintaining it within 0.2 °C of the target temperature. Other features of the TDT Sandwich include adjustable heating rates up to approximately 100 °C/min, real-time display and recording of temperature readings at a nominal rate of 5 Hz, an optional thermocouple for acquiring temperature of samples, built-in heating timer, and customizable operating parameters. The modular nature of the TDT Sandwich allows multiple units to be connected to a computer or laptop. Operation of the TDT Sandwich is done through a computer program which, along with the build instructions and microcontroller program, are open source and are available for free to the public at https://doi.org/10.17605/OSF.IO/5Q3Y7.
传统上，微生物热死亡动力学的测定是在液体浴中进行的，这种方法存在一些缺点，如液体溢出和液体渗入样品。TDT三明治作为一种免费的开源替代方案被开发出来，它利用干热。该系统能够将样品加热到140°C，并将其保持在目标温度的0.2°C范围内。TDT三明治的其他特点包括高达约100°C/分钟的可调加热速率、以5Hz的标称速率实时显示和记录温度读数、用于获取样品温度的可选热电偶、内置加热定时器以及可定制的操作参数。TDT三明治的模块化性质允许将多个单元连接到计算机或笔记本电脑。TDT三明治通过计算机程序进行操作，该程序连同构建说明和微控制器程序都是开源的，可在https://doi.org/10.17605/OSF.IO/5Q3Y7上免费向公众提供。
