Bassi Stefano, Galletti Giorgio, Carpana Emanuele, Palminteri Stefano, Bosi Filippo, Loglio Giulio, Carra Elena
Istituto Zooprofilattico Sperimentale della Lombardia e dell' Emilia Romagna "Bruno Ubertini", Brescia, Italy.
Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di ricerca Agricoltura e Ambiente, Bologna, Italy.
Front Vet Sci. 2022 Apr 14;9:853707. doi: 10.3389/fvets.2022.853707. eCollection 2022.
American Foulbrood (AFB) is a contagious and severe brood disease of honey bees caused by the spore-forming bacterium . The identification of honey bee colonies infected by is crucial for the effective control of AFB. We studied the possibility of identifying the infection levels by in honey bee colonies through the examination of powdered sugar samples collected in the hives. The powdered sugar was dusted on the top bars of honeycombs and collected from a sheet paper placed at the bottom of the hive. Three groups of honey bee colonies were examined: - colonies with clinical symptoms of AFB ( = 11); - asymptomatic colonies located in apiaries with colonies showing symptoms of AFB ( = 59); - asymptomatic colonies located in apiaries without cases of the disease ( = 49). The results showed that there was a significant difference in spore counting between and that the spore load in sugar samples was always consistent with the clinical conditions of the colonies and with their belonging to AFB-affected apiaries or not. Based on the obtained results the cultural examination of powdered sugar samples collected from hives could be an effective tool for the quantitative non-destructive assessment of infections in honey bee colonies.
美洲幼虫腐臭病（AFB）是一种由形成孢子的细菌引起的蜜蜂传染性严重幼虫病。识别感染该细菌的蜂群对于有效控制美洲幼虫腐臭病至关重要。我们研究了通过检查从蜂巢中收集的糖粉样本，来确定蜜蜂蜂群中该细菌感染水平的可能性。将糖粉撒在蜂巢的顶梁上，然后从放置在蜂巢底部的一张纸上收集。检查了三组蜜蜂蜂群：——有美洲幼虫腐臭病临床症状的蜂群（ = 11）；——位于有表现出美洲幼虫腐臭病症状蜂群的养蜂场中的无症状蜂群（ = 59）；——位于无该病病例的养蜂场中的无症状蜂群（ = 49）。结果表明，[此处原文缺失具体对比组]与[此处原文缺失具体对比组]之间的孢子计数存在显著差异，并且糖粉样本中的孢子载量始终与蜂群的临床状况以及它们是否属于受美洲幼虫腐臭病影响的养蜂场一致。基于所获得的结果，对从蜂巢中收集的糖粉样本进行培养检查可能是对蜜蜂蜂群中该细菌感染进行定量无损评估的有效工具。
