Kim S, Iwao H, Nakamura N, Ikemoto F, Yamamoto K
J Histochem Cytochem. 1987 May;35(5):549-57. doi: 10.1177/35.5.3549890.
We studied, by whole-body autoradiography, the distribution of exogenously administered renal renin in rat. Rat renal renin was completely purified and labeled with 125I ([125I]-renin) and was then injected into the tail veins of conscious rats at a dose of 30 microCi, 430 ng. After various intervals, rats were killed by an overdose of ether, the whole body rapidly frozen in acetone-dry ice, and autoradiography performed on sagittal whole-body sections. To remove breakdown products ([125I]-tyrosine and free 125I) from [125I]-renin, sections were treated with perchloric acid solution. The main accumulation of [125I]-renin acid-insoluble radioactivity was observed in liver and renal cortex. The accumulation in these organs was already evident 2 min after the injection, reached a maximum level by 15 min, then gradually decreased. A small amount of [125I]-renin was also evident in spleen, bone marrow, and adrenal gland. Thirty min after the injection, radioactivity began to appear in the thyroid gland, stomach, and small intestine, but disappeared with acid treatment, except in the thyroid. Radioactivity was negligible in other organs including brain, submaxillary gland, lung, heart, and testis. These autoradiographs clearly demonstrate that exogenously administered renal renin is distributed mainly in the liver and renal cortex.
我们通过全身放射自显影术研究了外源性给予大鼠的肾素在大鼠体内的分布情况。将大鼠肾素完全纯化并用¹²⁵I进行标记（[¹²⁵I]-肾素），然后以30微居里、430纳克的剂量注入清醒大鼠的尾静脉。在不同时间间隔后，用过量乙醚处死大鼠，将其全身迅速在丙酮 - 干冰中冷冻，然后对矢状全身切片进行放射自显影。为了从[¹²⁵I]-肾素中去除分解产物（[¹²⁵I]-酪氨酸和游离¹²⁵I），切片用高氯酸溶液处理。观察到[¹²⁵I]-肾素酸不溶性放射性主要积聚在肝脏和肾皮质中。注射后2分钟这些器官中的积聚就已明显，15分钟时达到最高水平，然后逐渐下降。在脾脏、骨髓和肾上腺中也有少量[¹²⁵I]-肾素。注射后30分钟，放射性开始出现在甲状腺、胃和小肠中，但经酸处理后除甲状腺外均消失。在包括脑、颌下腺、肺、心脏和睾丸在内的其他器官中放射性可忽略不计。这些放射自显影片清楚地表明，外源性给予的肾素主要分布在肝脏和肾皮质中。
