Jørgensen P E, Poulsen S S, Nexø E
Department of Urology D, Rigshospitalet, Copenhagen, Denmark.
Regul Pept. 1988 Nov;23(2):161-9. doi: 10.1016/0167-0115(88)90024-9.
The distribution of i.v. injected 125I-labeled epidermal growth factor (EGF) was examined in the rat. The uptake of radioactivity was examined for the following tissues: liver, kidney, skin, stomach, small intestine, colon, brain, submandibular gland, lung, spleen, and testis. 125I-EGF was cleared from the circulation within minutes. At 2.5 min after the injection only 7% of the label was left in the blood. Most of the label was found in the liver (52%), the kidneys (14%), the small intestine (11%) and the skin (7%). The other organs examined contained 1% or less of the radioactivity. The uptake of 125I-EGF per g tissue was markedly higher for the liver and kidneys than for the rest of the organs. By autoradiography 125I-EGF was found in the peripheral parts of the classical liver lobule, in the proximal tubules of the kidneys, in the surface epithelium of the stomach, and in the surface epithelium of the villi in the small intestine. In conclusion the present study showed that small doses of homologous EGF was cleared from the circulation of rats within minutes, mainly by the liver, the kidneys, and the small intestine.
在大鼠体内研究了静脉注射的125I标记表皮生长因子（EGF）的分布情况。检测了以下组织对放射性的摄取：肝脏、肾脏、皮肤、胃、小肠、结肠、脑、下颌下腺、肺、脾脏和睾丸。125I-EGF在数分钟内从循环中清除。注射后2.5分钟时，血液中仅留存7%的标记物。大部分标记物见于肝脏（52%）、肾脏（14%）、小肠（11%）和皮肤（7%）。所检测的其他器官含有的放射性不到1%。肝脏和肾脏每克组织对125I-EGF的摄取明显高于其他器官。通过放射自显影发现，125I-EGF存在于经典肝小叶的周边部分、肾脏的近端小管、胃的表面上皮以及小肠绒毛的表面上皮中。总之，本研究表明，小剂量的同源EGF在数分钟内从大鼠循环中清除，主要是通过肝脏、肾脏和小肠。
