Chai Zhaoliang, Bi Hongyan
College of Food Science and Engineering, Shanghai Ocean University, Hucheng Ring Road 999, Pudong New District, 201306 Shanghai, China.
Food Chem X. 2022 Jan 24;13:100225. doi: 10.1016/j.fochx.2022.100225. eCollection 2022 Mar 30.
In the present study, was taken as a model bacterium, anti- functionalized magnetic beads were constructed and used to capture from aqueous extracts of fish sarcoplasmic protein (FSP) and fish muscle protein of sablefish. The excellency of the reproducibility of the present protocol was demonstrated by capturing from sablefish FSP extracts. The presence of 10 CFU/mL is still detectable. A microbial safety test on the surface of fish muscle was successfully performed. The bacterial identification accuracy from samples with different matrices was found to be excellent with RSD = 3%. High specific detection of target bacteria in complex biological samples was testified by spiking and in samples as interference. Ten biomarker ions were discovered for 's recognition. It is promising to apply the present protocol in bacterial analysis in muscle food samples to ensure their safety.
在本研究中，以[具体细菌名称未给出]作为模式细菌，构建了抗功能化磁珠并用于从狭鳕鱼的鱼肌浆蛋白（FSP）和鱼肌肉蛋白的水提取物中捕获[具体细菌名称未给出]。通过从狭鳕鱼FSP提取物中捕获[具体细菌名称未给出]证明了本方案重现性的优异。10 CFU/mL[具体细菌名称未给出]的存在仍可检测到。成功地对鱼肌肉表面进行了微生物安全性测试。发现来自不同基质样品的细菌鉴定准确度极佳，相对标准偏差（RSD）=3%。通过在样品中加入[具体细菌名称未给出]和[干扰细菌名称未给出]作为干扰物，证实了在复杂生物样品中对目标细菌的高特异性检测。发现了十个用于识别[具体细菌名称未给出]的生物标志物离子。将本方案应用于肌肉食品样品的细菌分析以确保其安全性是有前景的。
