Capture and identification of bacteria from fish muscle based on immunomagnetic beads and MALDI-TOF MS.

In the present study, was taken as a model bacterium, anti- functionalized magnetic beads were constructed and used to capture from aqueous extracts of fish sarcoplasmic protein (FSP) and fish muscle protein of sablefish. The excellency of the reproducibility of the present protocol was demonstrated by capturing from sablefish FSP extracts. The presence of 10 CFU/mL is still detectable. A microbial safety test on the surface of fish muscle was successfully performed. The bacterial identification accuracy from samples with different matrices was found to be excellent with RSD = 3%. High specific detection of target bacteria in complex biological samples was testified by spiking and in samples as interference. Ten biomarker ions were discovered for 's recognition. It is promising to apply the present protocol in bacterial analysis in muscle food samples to ensure their safety.