College of Food Science and Engineering, Shanghai Ocean University, Hucheng Ring Road 999, Pudong New District, Shanghai 201306, China.
College of Food Science and Engineering, Shanghai Ocean University, Hucheng Ring Road 999, Pudong New District, Shanghai 201306, China.
Food Chem. 2022 Dec 1;396:133658. doi: 10.1016/j.foodchem.2022.133658. Epub 2022 Jul 9.
Bacterial contamination is a significant concern in food safety. Traditional methods, though being a gold standard for bacterial detection, are time-consuming. In this work, we managed to establish a simple and versatile magnetic-assisted microfluidic method for rapid bacterial detection of fish muscle products, by manipulating anti-human IgG functionalized magnetic beads in a zig-zag shaped microfluidic channel, increasing the probability for bacteria capture. The captured bacteria were characterized by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). This method is capable of isolating Escherichia coli, Staphylococcus aureus and Klebsiella pneumoniae from 5 μL of sablefish sarcoplasmic protein sample, and detecting Escherichia coli in the range of 6.0 to 6.0×10 CFU/mL with a detection limit of 6 CFU/mL. Bacterial growth on salmon sashimi during its period of storage was successfully monitored. The current protocol holds great potential for pathogen detection and microbial control in the food industry.
细菌污染是食品安全的一个重大问题。传统方法虽然是细菌检测的金标准，但耗时较长。在这项工作中，我们成功地建立了一种简单而通用的磁辅助微流控方法，用于快速检测鱼肉制品中的细菌，通过在曲折形微流道中操纵抗人 IgG 功能化的磁性珠，增加了细菌捕获的概率。捕获的细菌通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）进行表征。该方法能够从 5 μL 黑大麻哈鱼肉浆蛋白样品中分离出大肠杆菌、金黄色葡萄球菌和肺炎克雷伯菌，并能够检测到 6.0 至 6.0×10 CFU/mL 范围内的大肠杆菌，检测限为 6 CFU/mL。成功监测了三文鱼生鱼片在储存期间的细菌生长情况。该方案在食品工业中的病原体检测和微生物控制方面具有很大的潜力。
