Abedin Md Minhajul, Chourasia Rounak, Chiring Phukon Loreni, Singh Sudhir P, Kumar Rai Amit
Institute of Bioresources and Sustainable Development, Regional Centre, Tadong, Sikkim, India.
Center of Innovative and Applied Bioprocessing, SAS Nagar, Mohali, India.
Food Chem X. 2022 Jan 27;13:100231. doi: 10.1016/j.fochx.2022.100231. eCollection 2022 Mar 30.
In this study, simulated GI digestion of the Himalayan hard cheese resulted in the increase of hydrolyzed protein content, antioxidant and ACE-inhibitory activities. LC-MS/MS-based peptidomics revealed a total of 1473 peptides in the samples originating from different milk proteins, including α-S1-casein, α-S2-casein, β-casein, κ-casein, α-lactalbumin, and β-lactoglobulin, out of which 60 peptides have been reported for different functional properties. A total of 101 peptides were predicted to be antihypertensive using the bioactivity prediction web servers, AHTpin and mAHTPred. molecular docking studies predicted 20 antihypertensive peptides, exhibiting non-bond interactions between hard peptides and ACE catalytic residues. A peptide, SLVYPFPGPI, identified in GI digested cow hard and undigested, and GI digested samples of yak hard , showed a stronger binding affinity towards ACE. Identifying antioxidant and ACE inhibitory peptides in hard cheese products adds value to them as functional foods of the Himalayan region.
在本研究中，对喜马拉雅硬质奶酪进行模拟胃肠道消化后，水解蛋白含量、抗氧化活性和ACE抑制活性均有所增加。基于液相色谱-串联质谱的肽组学分析表明，样品中共有1473种源自不同乳蛋白的肽，包括α-S1-酪蛋白、α-S2-酪蛋白、β-酪蛋白、κ-酪蛋白、α-乳白蛋白和β-乳球蛋白，其中60种肽具有不同的功能特性。使用生物活性预测网络服务器AHTpin和mAHTPred预测，共有101种肽具有降压作用。分子对接研究预测了20种降压肽，显示出硬质肽与ACE催化残基之间的非键相互作用。在胃肠道消化的牛乳硬质奶酪、未消化的牦牛乳硬质奶酪以及胃肠道消化的牦牛乳硬质奶酪样品中均鉴定出的一种肽SLVYPFPGPI，对ACE表现出更强的结合亲和力。在硬质奶酪产品中鉴定出抗氧化和ACE抑制肽，使其作为喜马拉雅地区的功能性食品更具价值。
