Rao T D, Rao P R
Int J Lepr Other Mycobact Dis. 1987 Mar;55(1):36-41.
Cell-mediated immune (CMI) responses were measured in 39 lepromatous, 44 erythema nodosum leprosum (ENL), and 22 post-ENL patients. The leukocyte migration inhibition test was used to measure CMI responses to mitogen phytohemagglutinin-P (PHA), crossreacting antigen purified protein derivative (PPD) of tuberculin, and armadillo-derived whole and sonicate Mycobacterium leprae. "Early T" lymphocytes of the peripheral blood were also enumerated using the rosetting technique. Significantly enhanced immune responses (lower migratory indices) were found to whole M. leprae during ENL. Although responses were high with PHA and PPD, they were identical in all of the groups, indicating that during ENL reaction M. leprae-specific responses are enhanced. "Early T" lymphocytes also showed a significant increase in ENL reactions compared to lepromatous patients. However, there was no response to the leprolin skin test in ENL patients in contrast to the enhanced in vitro CMI responses.
对39例瘤型麻风、44例麻风结节性红斑（ENL）和22例ENL后患者进行了细胞介导免疫（CMI）反应检测。采用白细胞迁移抑制试验来检测CMI对丝裂原植物血凝素-P（PHA）、结核菌素交叉反应抗原纯化蛋白衍生物（PPD）以及犰狳源完整和超声处理的麻风分枝杆菌的反应。还使用玫瑰花结技术对外周血中的“早期T”淋巴细胞进行计数。发现在ENL期间，对完整麻风分枝杆菌的免疫反应显著增强（迁移指数降低）。尽管PHA和PPD的反应较高，但在所有组中均相同，这表明在ENL反应期间，麻风分枝杆菌特异性反应增强。与瘤型麻风患者相比，“早期T”淋巴细胞在ENL反应中也显著增加。然而，与体外增强的CMI反应形成对比的是，ENL患者对麻风菌素皮肤试验无反应。
