School of Public Health, Nantong University, Nantong, 226019, P. R. China.
Anal Chem. 2022 May 17;94(19):7115-7122. doi: 10.1021/acs.analchem.2c01041. Epub 2022 May 2.
In this work, a novel methodology to design bifunctional ECL-luminophores with self-enhanced and TSA-amplified characteristics was proposed for improving the sensing performance of ECL-immunosensor toward trace cytokine analysis. Thanks to the qualitative- and quantitative- dual signal amplification technique, the as-prepared ECL biosensor demonstrated excellent detection performance. By analyzing the prospective cytokine biomarkers (IL-6), the ECL immunosensor exhibited a broad examination range with quite low detection limit and quite high selectivity, which was far superior to commercial ELISA kits and ever reported works. In particular, the novel ECL nanoprobe developed here could also be applied to monitor other immune toxicities or disease-related cytokines by using the respective antibodies corresponding to these targets. Moreover, the concept and construction strategy of self-amplified ECL-luminophores presented here could be further extended to design a series of Pdots-derived multicolored ECL probes to meet the needs of multipathway detection applications.
在这项工作中，提出了一种新颖的方法来设计具有自增强和 TSA 放大特性的双功能 ECL 发光体，以提高 ECL 免疫传感器对痕量细胞因子分析的传感性能。由于定性和定量双重信号放大技术，所制备的 ECL 生物传感器表现出优异的检测性能。通过分析预期的细胞因子生物标志物（IL-6），ECL 免疫传感器具有较宽的检测范围，较低的检测限和较高的选择性，远远优于商业 ELISA 试剂盒和以往的报道工作。特别是，这里开发的新型 ECL 纳米探针还可以通过使用相应的针对这些靶标的抗体来监测其他免疫毒性或与疾病相关的细胞因子。此外，这里提出的自增强 ECL 发光体的概念和构建策略可以进一步扩展，以设计一系列基于 Pdots 的多色 ECL 探针，以满足多通路检测应用的需求。
