Zhang Yueyue, Hou Huixin, Cao Mengting, Zheng Yiyang, Wang Baozhu, Zhou Yuan, Qi Hang, Zhang Zhijun, Shen Yong-Miao
Key Laboratory of Surface & Interface Science of Polymer Materials of Zhejiang Province, School of Chemistry and Chemical Engineering, Zhejiang Sci-Tech University, Hangzhou, 310018, China.
Shengzhou Innovation Research Institute of Zhejiang Sci-Tech University, Shengzhou, 312400, China.
Mikrochim Acta. 2025 Jun 16;192(7):427. doi: 10.1007/s00604-025-07272-0.
In order to improve the stability, biocompatibility, and emissions of aqueous I-III-VI ternary quantum dots (QDs we report for the first time the one-pot two-step green synthesis method with proteins serving as modifier. As a proof of concept, bovine serum albumin (BSA) and lysozyme (Lys) modified CuInS@ZnS ternary core-shell QDs were synthesized respectively. The results indicate that proteins have no influence on the morphology, composition, and lattice of quantum dots. However, they can significantly increase the fluorescence intensity of quantum dots (by 1.5 times) and the fluorescence lifetime (by 3.5 times). In addition, protein modification sharply enhanced the stability of quantum dots in various media. Moreover, for the first time, the binding interaction between proteins and quantum dots was directly verified using the fluorescence quenching technique. Thereafter, the bacterial target and antibacterial function imparted to QDs by lysozyme were demonstrated. Finally, we verified the application potential of CIS/ZnS@Lys in different medical scenarios, which performed well in wound disinfection, imaging bacterial adhesion in medical catheters, and killing the adhered bacteria.
为了提高水性I-III-VI三元量子点（QDs）的稳定性、生物相容性和发射性能，我们首次报道了以蛋白质为改性剂的一锅两步绿色合成方法。作为概念验证，分别合成了牛血清白蛋白（BSA）和溶菌酶（Lys）修饰的CuInS@ZnS三元核壳量子点。结果表明，蛋白质对量子点的形貌、组成和晶格没有影响。然而，它们可以显著提高量子点的荧光强度（提高1.5倍）和荧光寿命（提高3.5倍）。此外，蛋白质修饰大大增强了量子点在各种介质中的稳定性。此外，首次使用荧光猝灭技术直接验证了蛋白质与量子点之间的结合相互作用。此后，证明了溶菌酶赋予量子点的细菌靶向和抗菌功能。最后，我们验证了CIS/ZnS@Lys在不同医学场景中的应用潜力，其在伤口消毒、医学导管中细菌粘附成像以及杀灭粘附细菌方面表现良好。
