Department of Neurology, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China.
Department of ICU, The First Affiliated Hospital of Hebei North University, Zhangjiakou, China.
J Clin Lab Anal. 2022 Jun;36(6):e24468. doi: 10.1002/jcla.24468. Epub 2022 May 2.
Long noncoding RNA intersectin 1-2 (lnc-ITSN1-2) regulates inflammation and neuronal apoptosis; meanwhile, the latter two factors participate in the pathogenesis of acute ischemic stroke (AIS). Therefore, this study detected lnc-ITSN1-2 at multiple time points, aiming to explore its longitudinal variation and clinical value in the management of AIS patients.
The current study enrolled 102 AIS patients, then detected their lnc-ITSN1-2 in peripheral blood mononuclear cell (PBMC) at baseline (D0), day (D)1, D3, D7, month (M)1, M3, M6, and year (Y)1 after admission using RT-qPCR. Additionally, lnc-ITSN1-2 in PBMC of 50 controls was also detected.
Lnc-ITSN1-2 was up-regulated in AIS patients than that in controls (p < 0.001). Lnc-ITSN1-2 positively associated with NIHSS score, TNF-α, and IL-17A (all p < 0.050) but was not linked with IL-6 (p = 0.093) in AIS patients. Notably, lnc-ITSN1-2 was gradually increased from D0 to D3; while it switched to decrease from D3 to Y1 in AIS patients. Lnc-ITSN1-2 disclosed similar longitudinal variation during 1 year in non-recurrent (p < 0.001), recurrent (p = 0.001), and survived patients (p < 0.001), while the variation of lnc-ITSN1-2 in died patients was not obvious (p = 0.132). More importantly, lnc-ITSN1-2 at D0, D3, D7, M1, M3, M6, and Y1 was higher in recurrent AIS patients than that in non-recurrent AIS patients (all p < 0.050); moreover, lnc-ITSN1-2 at D3, D7, M1, M3, and M6 was up-regulated in died AIS patients than AIS survivors (all p < 0.050).
The dynamic variation of Inc-ITSN1-2 could serve as a biomarker reflecting disease severity, inflammatory cytokines, recurrence, and death risk in AIS patients.
长链非编码 RNA intersectin 1-2（lnc-ITSN1-2）调节炎症和神经元凋亡；同时，后两者因素参与急性缺血性脑卒中（AIS）的发病机制。因此，本研究在多个时间点检测 lnc-ITSN1-2，旨在探讨其在 AIS 患者管理中的纵向变化和临床价值。
本研究纳入 102 例 AIS 患者，然后使用 RT-qPCR 在基线（D0）、第 1 天（D1）、第 3 天（D3）、第 7 天（D7）、第 1 个月（M1）、第 3 个月（M3）、第 6 个月（M6）和第 1 年（Y1）时检测其外周血单核细胞（PBMC）中的 lnc-ITSN1-2。此外，还检测了 50 名对照者 PBMC 中的 lnc-ITSN1-2。
AIS 患者的 lnc-ITSN1-2 高于对照组（p<0.001）。AIS 患者中，lnc-ITSN1-2 与 NIHSS 评分、TNF-α 和 IL-17A 呈正相关（均 p<0.050），但与 IL-6 无相关性（p=0.093）。值得注意的是，lnc-ITSN1-2 在 AIS 患者中从 D0 到 D3 逐渐升高，从 D3 到 Y1 则呈下降趋势。在非复发（p<0.001）、复发（p=0.001）和存活（p<0.001）患者中，lnc-ITSN1-2 在 1 年内也呈现出相似的纵向变化，而在死亡患者中（p=0.132），lnc-ITSN1-2 的变化并不明显。更重要的是，复发 AIS 患者在 D0、D3、D7、M1、M3、M6 和 Y1 时的 lnc-ITSN1-2 均高于非复发 AIS 患者（均 p<0.050），而在死亡 AIS 患者中，在 D3、D7、M1、M3 和 M6 时的 lnc-ITSN1-2 均高于 AIS 存活者（均 p<0.050）。
lnc-ITSN1-2 的动态变化可作为反映 AIS 患者疾病严重程度、炎症细胞因子、复发和死亡风险的生物标志物。
