College of Environmental Science and Engineering, Donghua University, 2999 North Renmin Road, Shanghai 201620, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.
College of Environmental Science and Engineering, Donghua University, 2999 North Renmin Road, Shanghai 201620, China.
J Hazard Mater. 2022 Aug 5;435:128944. doi: 10.1016/j.jhazmat.2022.128944. Epub 2022 Apr 20.
Peroxydisulfate (PDS) is widely used in field-scale remediation of organically polluted soil, yet PDS is detrimental to the soil microbiome. In this work, sludge-derived hydrochar was used to alleviate the oxidative damage of PDS to the soil ecosystem and simultaneously improve atrazine (ATZ) degradation. Response surfaces showed that ATZ degradation was enhanced with an increased dosage of PDS (regression slope of 24.09) and hydrochar (regression slope of 4.19). In contrast, bacterial abundance was negatively related to PDS dosage but positively to hydrochar dosage. At the optimum dosage of PDS (2.21% dry weight of soil) and hydrochar (5% dry weight of soil), ATZ degradation reached 95.31%, and bacterial abundance recovered to 7.72 log gene copy number g soil (versus 8.44 in raw soil). Hydrochar alleviated the negative impacts of PDS on soil fertility such as urease activity. High PDS dosages (3% dry weight of soil) facilitated the proliferation of Halomonas, while moderate dosages (1.5% dry weight of soil) stimulated Alicyclobacillus. Hydrochar facilitated the growth of functional genera like Comamonas, Cloacibacterium, and Terrabacter. ATZ degradation pathway was positively correlated with Bacillus and nitrogen metabolism pathway. Hydrochar mediated intracellular reactive oxidative species scavenger reactions in catalase activity, allowing microbial survival under harsh oxidative conditions due to PDS addition.
过二硫酸盐（PDS）广泛应用于有机污染土壤的现场修复，但 PDS 会损害土壤微生物组。在这项工作中，采用污泥衍生的水热炭来缓解 PDS 对土壤生态系统的氧化损伤，同时提高莠去津（ATZ）的降解。响应面表明，随着 PDS（回归斜率为 24.09）和水热炭（回归斜率为 4.19）用量的增加，ATZ 的降解得到了增强。相比之下，细菌丰度与 PDS 用量呈负相关，而与水热炭用量呈正相关。在 PDS（土壤干重的 2.21%）和水热炭（土壤干重的 5%）的最佳用量下，ATZ 的降解率达到 95.31%，细菌丰度恢复到 7.72 log 基因拷贝数 g 土壤（与原始土壤中的 8.44 相比）。水热炭缓解了 PDS 对土壤肥力的负面影响，如脲酶活性。高 PDS 用量（土壤干重的 3%）促进了盐单胞菌的增殖，而中等用量（土壤干重的 1.5%）则刺激了环状芽孢杆菌的增殖。水热炭促进了功能属如贪噬菌属、粪球菌属和土壤杆菌属的生长。ATZ 降解途径与芽孢杆菌和氮代谢途径呈正相关。水热炭介导了过氧化氢酶活性中的细胞内活性氧化物质清除反应，使得微生物能够在添加 PDS 后在恶劣的氧化条件下存活。
