Institute of Agricultural Resources and Environment, Jilin Academy of Agricultural Sciences, 1363 Shengtai Street, Changchun, 130033, China.
Institute of Agricultural Resources and Environment, Jilin Academy of Agricultural Sciences, 1363 Shengtai Street, Changchun, 130033, China.
Chemosphere. 2021 Nov;282:130976. doi: 10.1016/j.chemosphere.2021.130976. Epub 2021 May 28.
Atrazine is one of the most widely used herbicides, however it and its metabolites cause widespread contamination in soil and ground water. Bioaugmentation is an effective method for remediation of environmental organic pollutants. High-throughput sequencing provides an important tool for understanding the changes of microbial community and function in response to pollutants degradation based on bioaugmentation. In this study, the effect of biodegradation with Paenarthrobacter sp. W11 and the change of microbial community during atrazine degradation were investigated. The results showed that bioaugmentation significantly accelerated the degradation rate of atrazine in soil and reduced the toxic effect of atrazine residues on wheat growth. The extra available NH through atrazine mineralization could serve as a nitrogen source to increase microbial numbers. High-throughput sequencing further revealed that the microbial community restored a new balance. The function of microbial community predicted by PICRUSt2 suggested that the biodegradation process of atrazine affected not only the atrazine degradation pathway, but also the nitrogen metabolism pathway. Methylobacillus and Pseudomonas were considered as the most important indigenous atrazine-degrading microorganisms, because their relative abundances were positively correlated with the relative abundance of Paenarthrobacter and atrazine degradation pathway. This study provides insight into the cooperation between indigenous microorganisms and external inoculums on atrazine degradation process.
莠去津是一种应用广泛的除草剂，但它及其代谢物会导致土壤和地下水的广泛污染。生物强化是修复环境有机污染物的有效方法。高通量测序为了解基于生物强化的微生物群落和功能对污染物降解的响应变化提供了重要工具。本研究考察了莠去津降解过程中 Paenarthrobacter sp. W11 的生物降解效果及微生物群落的变化。结果表明，生物强化显著加速了土壤中莠去津的降解速率，降低了莠去津残留对小麦生长的毒害作用。莠去津矿化产生的额外有效 NH 可为微生物提供氮源，从而增加微生物数量。高通量测序进一步揭示了微生物群落恢复了新的平衡。PICRUSt2 预测的微生物群落功能表明，莠去津的生物降解过程不仅影响莠去津降解途径，还影响氮代谢途径。Methylobacillus 和 Pseudomonas 被认为是最重要的土著莠去津降解微生物，因为它们的相对丰度与 Paenarthrobacter 和莠去津降解途径的相对丰度呈正相关。本研究深入了解了土著微生物与外源接种物在莠去津降解过程中的协同作用。
