Faculty of Biology, M. V. Lomonosov Moscow State University, Moscow, Russia.
University Diagnostic Laboratory, Moscow, Russia.
Bull Exp Biol Med. 2022 Apr;172(6):734-737. doi: 10.1007/s10517-022-05467-6. Epub 2022 May 2.
Lichen sclerosus of the vulva is a common, but poorly studied disease. We assessed the level of transcriptional activity of APAF1, BAX, BCL2, BIRC5, CCND1, DAPK1, MCL1, and MYC genes encoding products that control apoptosis in the samples of tissues affected by vulvar lichen sclerosus and adjacent control tissues (n=24). Analysis of transcriptional activity was performed by real-time PCR using specific primers and SYBR Green intercalating dye. After the total group was divided by the presence of the concomitant gynecological diseases, a significant increase in the transcriptional activity of the CCND1 gene was revealed in patients with concomitant uterine fibroids. This may indicate the possible role of the activation of mitosis during tumor initiation.
外阴硬化性苔藓是一种常见但研究不足的疾病。我们评估了编码凋亡相关蛋白因子 1（apoptosis-associated factor 1，APAF1）、B 淋巴细胞瘤-2 相关 X 蛋白（B-cell lymphoma-2 associated X protein，BAX）、B 细胞淋巴瘤-2（B-cell lymphoma-2，BCL2）、凋亡抑制蛋白 5（inhibitor of apoptosis protein 5，BIRC5）、周期素 D1（cyclin D1，CCND1）、死亡相关蛋白激酶 1（death-associated protein kinase 1，DAPK1）、髓系细胞白血病-1（myeloid cell leukemia-1，MCL1）和 MYC 基因在受外阴硬化性苔藓影响的组织样本和相邻对照组织（n=24）中的转录活性水平。使用特异性引物和 SYBR Green 嵌入染料通过实时 PCR 进行转录活性分析。在根据是否存在妇科合并症对总组进行分组后，发现在同时患有子宫肌瘤的患者中，CCND1 基因的转录活性显著增加。这可能表明在肿瘤起始时有丝分裂激活的可能作用。
