Research Institute of General Pathology and Pathophysiology, Moscow, Russia.
Federal Research Clinical Center of Specialized Types of Medical Care and Medical Technologies, Federal Medical-Biological Agency of Russia, Moscow, Russia.
Bull Exp Biol Med. 2022 Apr;172(6):760-764. doi: 10.1007/s10517-022-05473-8. Epub 2022 May 2.
Late diagnosis of ovarian cancer is one of the most important problems in its treatment. Long non-coding RNA (lncRNA) are a poorly studied, but promising type of diagnostic biomarkers. We studied the lncRNA interactome to identify biomarkers with potential significance for molecular diagnostics of ovarian cancer. By screening the TCGA database, we identified differentially expressed lncRNA CCAT1 and SNHG14. Based on the indices of complementarity of CCAT1 and SNHG14 to the mRNA sequences, we selected 5 protein-coding genes MAPK1, c-MET, TGFB2, SNAIL1, and WNT4 associated with the epithelial-mesenchymal transition. Real-time PCR on 54 ovarian cancer samples confirmed the high expression levels of CCAT1 and SNHG14 (logFC>1.5, p<0.05). A positive correlation between the expression levels of two lncRNA and mRNA of 5 genes in 6 pairs was established. The activating effect of CCAT1 and SNHG14 on the expression of these genes can be mediated by miR-203 and miR-124.
卵巢癌的晚期诊断是其治疗中最重要的问题之一。长链非编码 RNA（lncRNA）是一种研究较少但很有前途的诊断生物标志物类型。我们研究了 lncRNA 相互作用组，以确定具有卵巢癌分子诊断潜在意义的生物标志物。通过筛选 TCGA 数据库，我们鉴定出差异表达的 lncRNA CCAT1 和 SNHG14。基于 CCAT1 和 SNHG14 与 mRNA 序列互补性的指标，我们选择了与上皮间质转化相关的 5 个蛋白编码基因 MAPK1、c-MET、TGFB2、SNAIL1 和 WNT4。对 54 个卵巢癌样本进行实时 PCR 验证了 CCAT1 和 SNHG14 的高表达水平（logFC>1.5，p<0.05）。在 6 对中建立了两个 lncRNA 和 5 个基因的 mRNA 表达水平之间的正相关关系。CCAT1 和 SNHG14 对这些基因表达的激活作用可以通过 miR-203 和 miR-124 来介导。
