Department of Clinical Medicine, College of Medicine, Pingdingshan University, Pingdingshan, China.
Department of Gastroenterology, First Affiliated Hospital of Pingdingshan University, Pingdingshan, China.
Geriatr Gerontol Int. 2020 Jun;20(6):629-636. doi: 10.1111/ggi.13914. Epub 2020 Mar 30.
Lung cancer serves as one of the most common cancers in the world, and approximately 50% of non-small-cell lung cancer (NSCLC) patients are found to be aged >70 when diagnosed. In this study, we aimed to explore the effect of long non-coding RNAs colon cancer-associated transcript-1 (CCAT1) in NSCLC.
A total of 72 clinical samples from older NSCLC patients were collected for analysis. The relative mRNA level of CCAT1 was detected through real-time polymerase chain reaction. Overall survival of NSCLC patients was detected through Kaplan-Meier survival analysis. MTT assays were used to detect cell proliferation. Cell invasion was determined by transwell assay. Protein levels were detected through western blot.
CCAT1 expression levels significantly increased in NSCLC tumor tissues and were associated with poor overall survival of NSCLC patients. CCAT1 promotes cell proliferation, cell invasion and epithelial-mesenchymal transition of NSCLC cell lines. CCAT1 binds with miR-152, and the effect of si-CCAT1 in NSCLC cell proliferation, cell invasion and epithelial-mesenchymal transition was partially reversed by anti-miR-152.
Long non-coding RNA CCAT1 regulates tumor cell proliferation and invasion in NSCLC through suppressing miR-152. Geriatr Gerontol Int 2020; ••: ••-••.
肺癌是世界上最常见的癌症之一，大约 50%的非小细胞肺癌（NSCLC）患者在诊断时年龄>70 岁。在这项研究中，我们旨在探讨长链非编码 RNA 结肠癌相关转录物-1（CCAT1）在 NSCLC 中的作用。
共收集了 72 例老年 NSCLC 患者的临床样本进行分析。通过实时聚合酶链反应检测 CCAT1 的相对 mRNA 水平。通过 Kaplan-Meier 生存分析检测 NSCLC 患者的总生存情况。MTT 法检测细胞增殖。Transwell 测定法检测细胞侵袭。通过 Western blot 检测蛋白水平。
CCAT1 在 NSCLC 肿瘤组织中的表达水平显著升高，与 NSCLC 患者的总生存不良相关。CCAT1 促进 NSCLC 细胞系的细胞增殖、细胞侵袭和上皮-间充质转化。CCAT1 与 miR-152 结合，si-CCAT1 在 NSCLC 细胞增殖、细胞侵袭和上皮-间充质转化中的作用部分被抗 miR-152 逆转。
长链非编码 RNA CCAT1 通过抑制 miR-152 调节 NSCLC 肿瘤细胞的增殖和侵袭。老年医学与老年病学国际 2020 年；••：••-••。
