Department of Chemistry, Gazi University, 06500, Ankara, Turkey.
Department of Biology, Karamanoglu Mehmetbey University, 70200, Karaman, Turkey.
Biol Trace Elem Res. 2023 Apr;201(4):2058-2070. doi: 10.1007/s12011-022-03264-w. Epub 2022 Apr 30.
Rare earth elements have shown promising results in both bio-imaging and therapy applications due to their superior magnetic, catalytic, and optical properties. In recent years, since lanthanide-based nanomaterials have effective results in wound healing, it has become necessary to investigate the different properties of these nanoparticles. The aim of this study is to investigate the antimicrobial, antibiofilm, and biocompability of Eu(OH) and Tb(OH) nanorods, which have a high potential by triggering angiogenesis and providing ROS activity, especially in wound healing. For this purpose, nanorods were obtained by the microwave-assisted synthesis method. Structural characterizations of Eu(OH) and Tb(OH) nanorods were performed by FT-IR, XRD, and TG-DTA methods, and morphological characterizations were performed by SEM-EDX. Microorganisms that are likely to be present in the wound environment were selected for the antimicrobial activities of the nanorods. The highest efficiency of nanorods with the disc diffusion method was shown against Pseudomonas aeruginosa ATCC 27,853 and Candida albicans ATCC 10,231 microorganisms. One of the problems frequently encountered in an infected wound environment is the formation of bacterial biofilm. Eu(OH) nanorods inhibited 77.5 ± 0.43% and Tb(OH) nanorods 76.16 ± 0.60% of Pseudomonas aeruginosa ATCC 27,853 biofilms. These results show promise for the development of biomaterials with superior properties by adding these nanorods to wound dressings that will be developed especially for wounds with microbial infection. Eu(OH) nanorods are more toxic than Tb(OH) nanorods on NCTC L929 cells. At concentrations of 500 µg/ml and above, both nanorods are toxic to cells.
稀土元素由于其优异的磁性、催化和光学性能，在生物成像和治疗应用中显示出有前景的结果。近年来，由于镧系纳米材料在伤口愈合方面具有显著效果，因此有必要研究这些纳米粒子的不同特性。本研究旨在研究 Eu(OH)和 Tb(OH)纳米棒的抗菌、抗生物膜和生物相容性，这些纳米棒具有通过触发血管生成和提供 ROS 活性的高潜力，特别是在伤口愈合方面。为此，通过微波辅助合成法获得了纳米棒。通过 FT-IR、XRD 和 TG-DTA 方法对 Eu(OH)和 Tb(OH)纳米棒的结构进行了表征，通过 SEM-EDX 对形貌进行了表征。选择可能存在于伤口环境中的微生物来评估纳米棒的抗菌活性。纳米棒的圆盘扩散法对铜绿假单胞菌 ATCC 27,853 和白色念珠菌 ATCC 10,231 微生物的效率最高。在感染性伤口环境中经常遇到的问题之一是细菌生物膜的形成。Eu(OH)纳米棒抑制了 77.5±0.43%的铜绿假单胞菌 ATCC 27,853 生物膜，Tb(OH)纳米棒抑制了 76.16±0.60%的生物膜。这些结果表明，通过将这些纳米棒添加到特别是针对微生物感染伤口开发的伤口敷料中，可以开发出具有优异性能的生物材料。Eu(OH)纳米棒对 NCTC L929 细胞的毒性比 Tb(OH)纳米棒大。在 500μg/ml 及以上浓度下，两种纳米棒对细胞均有毒性。
