Biological Activities and Biocompatibility Properties of Eu(OH) and Tb(OH) Nanorods: Evaluation for Wound Healing Applications.

Rare earth elements have shown promising results in both bio-imaging and therapy applications due to their superior magnetic, catalytic, and optical properties. In recent years, since lanthanide-based nanomaterials have effective results in wound healing, it has become necessary to investigate the different properties of these nanoparticles. The aim of this study is to investigate the antimicrobial, antibiofilm, and biocompability of Eu(OH) and Tb(OH) nanorods, which have a high potential by triggering angiogenesis and providing ROS activity, especially in wound healing. For this purpose, nanorods were obtained by the microwave-assisted synthesis method. Structural characterizations of Eu(OH) and Tb(OH) nanorods were performed by FT-IR, XRD, and TG-DTA methods, and morphological characterizations were performed by SEM-EDX. Microorganisms that are likely to be present in the wound environment were selected for the antimicrobial activities of the nanorods. The highest efficiency of nanorods with the disc diffusion method was shown against Pseudomonas aeruginosa ATCC 27,853 and Candida albicans ATCC 10,231 microorganisms. One of the problems frequently encountered in an infected wound environment is the formation of bacterial biofilm. Eu(OH) nanorods inhibited 77.5 ± 0.43% and Tb(OH) nanorods 76.16 ± 0.60% of Pseudomonas aeruginosa ATCC 27,853 biofilms. These results show promise for the development of biomaterials with superior properties by adding these nanorods to wound dressings that will be developed especially for wounds with microbial infection. Eu(OH) nanorods are more toxic than Tb(OH) nanorods on NCTC L929 cells. At concentrations of 500 µg/ml and above, both nanorods are toxic to cells.