Oyaide K, Sono A, Kobayashi Y, Yamamoto H
Jpn J Antibiot. 1986 Dec;39(12):3349-52.
Mutation frequency tests for isepamicin (HAPA-B), a new aminoglycoside antibiotic, were carried out using Salmonella typhimurium strains TA98, TA100, TA1535 and Escherichia coli WP2uvrA. After a 30-minute exposure of bacterial cells at 37 degrees C to HAPA-B in a buffer solution, the cells were washed twice with a buffer solution to minimize the cytotoxicity of the drug. Then washed cells were inoculated on minimum glucose agar plates to determine mutation frequency. The results should no significant increase in mutation frequency with increasing HAPA-B concentration; suggesting that the drug has no mutagenicity detectable by this test system.
使用鼠伤寒沙门氏菌TA98、TA100、TA1535菌株和大肠杆菌WP2uvrA对新的氨基糖苷类抗生素异帕米星（HAPA-B）进行了突变频率测试。在37℃下将细菌细胞在缓冲溶液中暴露于HAPA-B 30分钟后，用缓冲溶液洗涤细胞两次，以尽量减少药物的细胞毒性。然后将洗涤后的细胞接种在最低葡萄糖琼脂平板上以确定突变频率。结果显示，随着HAPA-B浓度的增加，突变频率没有显著增加；这表明该药物在此测试系统中没有可检测到的致突变性。
