Ohino R, Ueda R, Imai Kato Y, Watanabe E, Morishima Y, Yokomaku S, Kobayashi M, Takeyama H, Ezaki K, Kawashima K, Hirano M, Ohara K, Kosaki T, Yoshikawa S, Yamada K
Gan. 1978 Apr;69(2):179-86.
A clinical trial of chemoimmunotherapy using cell-wall skeleton of BCG (BCG-CWS) was conducted in 28 patients with acute leukemia in complete remission. Chemotherapy consisted of monthly intensification therapy for 2 months and bimonthly therafter. Immunotherapy with 200 microgram of oil-attached BCG-CWS mixed with 10(7) autochthonous leukemic cells was given intradermally at either of upper or lower extremities every week, except when the patients were on maintenance therapy. No serious systemic side effect attributable to BCG-CWS was noted. Six patients developed mild and transient temparature elevation. Most importnat side effect was local skin reaction. Indulated papules developed in all patients, resulting in draining ulcerations in 26 patients. Increase of immunological reactivity of the patients receiving BCG-CWS was noted. Skin test response to PPD, Varidase, and candida extract showed definite increase. In vitro lymphocyte blastogenic response to PPD, concanavalin-A, and pokeweed mitogen revealed significant increase.
对28例急性白血病完全缓解患者进行了使用卡介苗细胞壁骨架（BCG - CWS）的化学免疫疗法临床试验。化疗包括每月强化治疗2个月，之后每两个月进行一次。除患者进行维持治疗时外，每周在上下肢任一部位皮内注射200微克与10⁷自体白血病细胞混合的油剂卡介苗细胞壁骨架进行免疫治疗。未观察到归因于BCG - CWS的严重全身副作用。6例患者出现轻度短暂体温升高。最主要的副作用是局部皮肤反应。所有患者均出现硬结丘疹，26例患者出现引流性溃疡。观察到接受BCG - CWS患者的免疫反应性增加。对PPD、链激酶和念珠菌提取物的皮肤试验反应有明显增加。体外淋巴细胞对PPD、刀豆球蛋白A和商陆有丝分裂原的增殖反应显著增加。
