Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China; The Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China.
Colloids Surf B Biointerfaces. 2022 Jul;215:112527. doi: 10.1016/j.colsurfb.2022.112527. Epub 2022 Apr 28.
Colorimetric or fluorescent biosensors based on mimic enzymes have come into the spotlight in virtue of their visual detection. In traditional visual sensors, fluorescent-changing or color-changing substances should be introduced for the catalytic reaction with mimic enzymes. Herein, a mimic enzyme (Au@Fe-MIL-88B) with self-triggered fluorescent property was prepared. By incorporating Au nanoparticles (Au NPs) in Fe-MIL-88B, a higher peroxidase activity of Au@Fe-MIL-88B was monitored due to the synergistic effect between Au NPs and Fe-MIL-88B. Besides, Au NPs can change the valence of Fe ion in metal organic framework (MOF), thus lower background fluorescence was discovered, but the addition of HO can trigger the self-fluorescence of Au@Fe-MIL-88B. By using Au@Fe-MIL-88B as a label to anchor secondary antibody, a competitive immunosensor based on fluorescence and photoelectrochemistry was constructed for the immunoassay of rosiglitazone (RSG), a kind of hypoglycemic drug. Finally, a portable instrument was homemade for the on-site and convenient detection of RSG in functional tea. This self-triggered fluorescent MOF may provide a possible route to design biosensors for the detection of hazardous materials.
基于模拟酶的比色或荧光生物传感器因其可视化检测而受到关注。在传统的视觉传感器中，应该引入荧光变化或颜色变化的物质，以与模拟酶进行催化反应。在此，制备了具有自触发荧光特性的模拟酶（Au@Fe-MIL-88B）。通过将金纳米粒子（Au NPs）掺入 Fe-MIL-88B 中，由于 Au NPs 和 Fe-MIL-88B 之间的协同作用，监测到 Au@Fe-MIL-88B 的过氧化物酶活性更高。此外，Au NPs 可以改变金属有机骨架（MOF）中 Fe 离子的价态，从而发现背景荧光降低，但添加 HO 可以触发 Au@Fe-MIL-88B 的自荧光。通过将 Au@Fe-MIL-88B 用作标记物来固定二级抗体，构建了基于荧光和光电化学的竞争性免疫传感器，用于检测罗格列酮（RSG），一种降血糖药物。最后，自制了一种便携式仪器，用于在功能茶中现场和方便地检测 RSG。这种自触发荧光 MOF 可能为设计用于检测有害物质的生物传感器提供了一种可能的途径。
