Provincial Key Laboratory of Rural Energy Engineering in Yunnan, Yunnan Normal University, Kunming 650500, China.
School of Energy and Environment Science, Yunnan Normal University, Kunming 650500, China.
Biosensors (Basel). 2023 Aug 25;13(9):846. doi: 10.3390/bios13090846.
Metal-organic frameworks (MOFs) are often used as carriers in the preparation of electrochemiluminescent (ECL) materials, and ECL materials stabilized in the aqueous phase can be prepared by encapsulating chromophores inside MOFs by an in situ growth method. In this study, nanocomposites MIL-88B(Fe)-NH@Ru(py) with excellent ECL response were prepared by encapsulating Tris(2,2'-bipyridine)ruthenium dichloride (Ru(py)) inside MIL-88B(Fe)-NH using the one-step hydrothermal method. MIL-88B(Fe)-NH possesses abundant amino groups, which can accelerate the catalytic activation process of KSO, and its abundant pores are also conducive to the enhancement of the transmission rate of co-reactant agents, ions, and electrons, which effectively improves the ECL efficiency. In order to obtain more excellent ECL signals, we prepared aminated biochar (NH-biochar) using Pu-erh tea dregs as precursor and loaded gold nanoparticles (Au NPs) on its surface as substrate material for modified electrodes. Both NH-biochar and Au NPs can also be used as a co-reactant promoter to catalyze the activation process of co-reactant KSO. Therefore, a sandwich-type ECL immunosensor was prepared based on a dual signal-enhanced strategy for the highly sensitive and selective detection of aflatoxin B1 (AFB1). Under the optimal experimental conditions, the sensitive detection of AFB1 was achieved in the range of 1 pg·mL~100 ng·mL with a detection limit of 209 fg·mL. The proposed dual signal-enhanced ECL immunosensor can provide a simple, convenient, and efficient method for the sensitive detection of AFB1 in food and agricultural products.
金属-有机骨架（MOFs）通常被用作制备电致化学发光（ECL）材料的载体，通过原位生长法将发色团封装在 MOFs 中，可以制备在水相稳定的 ECL 材料。本研究采用一步水热法，将三（2,2'-联吡啶）钌二氯化物（Ru(py)）封装在 MIL-88B(Fe)-NH 内，制备了具有优异 ECL 响应的纳米复合材料 MIL-88B(Fe)-NH@Ru(py)。MIL-88B(Fe)-NH 具有丰富的氨基，可加速 KSO 的催化活化过程，其丰富的孔道也有利于增强共反应物、离子和电子的传输速率，从而有效提高 ECL 效率。为了获得更优异的 ECL 信号，我们以普洱茶渣为前驱体制备了氨基化生物炭（NH-biochar），并在其表面负载金纳米粒子（Au NPs）作为修饰电极的基底材料。NH-biochar 和 Au NPs 均可作为共反应物促进剂，促进共反应物 KSO 的活化过程。因此，基于双信号增强策略，制备了三明治型 ECL 免疫传感器，用于高灵敏和选择性检测黄曲霉毒素 B1（AFB1）。在最佳实验条件下，实现了在 1 pg·mL~100 ng·mL 范围内对 AFB1 的灵敏检测，检测限为 209 fg·mL。所提出的双信号增强 ECL 免疫传感器为食品和农产品中 AFB1 的灵敏检测提供了一种简单、方便、高效的方法。
