采用超高效液相色谱-三重四极杆串联质谱法（UPLC-QqQ-MS/MS）同时定量测定杜仲及其茶制品中的 14 种特征活性化合物。
Simultaneous quantification of fourteen characteristic active compounds in Eucommia ulmoides Oliver and its tea product by ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-QqQ-MS/MS).
机构信息
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, PR China; Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry-based Active Substances, Harbin 150040, PR China.
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; College of Forestry, Beijing Forestry University, Beijing 100083, PR China.
出版信息
Food Chem. 2022 Sep 30;389:133106. doi: 10.1016/j.foodchem.2022.133106. Epub 2022 Apr 29.
Various kinds of bioactive compounds contribute to versatile health-promoting properties of Eucommia ulmoides Oliver (E. ulmoides). In present study, we developed a UPLC-QqQ-MS/MS method for simultaneous quantification of fourteen characteristic active compounds, including 3 lignans, 4 iridoids, 3 flavonoids and 4 phenolics in E. ulmoides and its tea product for the first time. The running time of the method is 6.5 min. It has good linearity, sensitivity, precision, accuracy, and stability. Using this high-throughput method, the distributions of fourteen characteristic active compounds in E. ulmoides and its tea product were clarified. Also, it was found that E. ulmoides tea exhibited superiority in contents of chlorogenic acid as compared with natural resources. Overall, the study provided a rapid, reliable, and efficient analysis method, which could be applied for the quality evaluation of E. ulmoides natural resources and their relative products in the field of food and medicine.
各种生物活性化合物赋予杜仲（Eucommia ulmoides Oliver）多种促进健康的特性。在本研究中，我们首次建立了一种超高效液相色谱-三重四极杆串联质谱法（UPLC-QqQ-MS/MS），用于同时定量分析杜仲及其茶产品中的 14 种特征性活性化合物，包括 3 种木脂素、4 种环烯醚萜、3 种黄酮类和 4 种酚类化合物。该方法的运行时间为 6.5 分钟。它具有良好的线性、灵敏度、精密度、准确性和稳定性。使用这种高通量方法，阐明了 14 种特征性活性化合物在杜仲及其茶产品中的分布情况。此外，还发现杜仲茶中绿原酸的含量优于天然资源。总的来说，该研究提供了一种快速、可靠、有效的分析方法，可应用于食品和医药领域中杜仲天然资源及其相关产品的质量评价。
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