Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, People's Republic of China.
J Ind Microbiol Biotechnol. 2010 May;37(5):447-54. doi: 10.1007/s10295-010-0690-0. Epub 2010 Feb 2.
Eucommia ulmoides Oliver is a traditional medicinal plant of China, and it is one of the main sources of chlorogenic acid. Chlorogenic acid is an ester of caffeic acid, quinic acid, and a phenolic compound that has antibacterial, antifungal, antioxidant, and antitumor activities. The purpose of this study was to determine whether endophytic fungi isolated from Eucommia ulmoides Oliver had the same ability to produce chlorogenic acid. Primary screening was done by antibacterial and antifungal reactions, and the strain reselection was done with high-performance liquid chromatography (HPLC) to identify the fermentation products of the selected strains. Extracts of the leaf and cortex of Eucommia ulmoides Oliver were also deteted by HPLC, then positive results of HPLC were analyzed by GC-MS and LC-MS. In this study, 29 strains were isolated from Eucommia ulmoides Oliver. Most of them had antibacterial activity, and a few of them had antifungal activity. One ingredient of the B5 extract had a retention time identical to that of authentic chlorogenic acid. With GC-MS, other ingredients, isocoumarin and p-chlorocinnamide, were found. With LC-MS, chlorogenic acid and geniposide related to Eucommia ulmoides Oliver were found. The strain B5 was identified as Sordariomycete sp. Thus, endophytic fungi may produce the bioactive compound chlorogenic acid, as their host plant does, and could be used for the production of chlorogenic acid by fermentation in the future.
杜仲是中国的传统药用植物，也是绿原酸的主要来源之一。绿原酸是咖啡酸、奎宁酸和酚类化合物的酯，具有抗菌、抗真菌、抗氧化和抗肿瘤活性。本研究旨在确定从杜仲中分离出的内生真菌是否具有产生绿原酸的相同能力。通过抗菌和抗真菌反应进行初步筛选，然后用高效液相色谱（HPLC）对选定菌株进行再选择，以鉴定所选菌株的发酵产物。还通过 HPLC 检测了杜仲的叶和皮提取物，然后对 HPLC 的阳性结果进行 GC-MS 和 LC-MS 分析。在这项研究中，从杜仲中分离出 29 株内生真菌。它们大多数具有抗菌活性，少数具有抗真菌活性。B5 提取物的一种成分的保留时间与真实绿原酸的保留时间相同。通过 GC-MS 发现了其他成分，即异香豆素和对氯肉桂酰胺。通过 LC-MS 发现了与杜仲有关的绿原酸和京尼平苷。菌株 B5 被鉴定为 Sordariomycetes sp. 因此，内生真菌可能像其宿主植物一样产生具有生物活性的绿原酸化合物，并可在未来通过发酵用于绿原酸的生产。
