Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, Fuchu, Tokyo, 183-8509, Japan.
Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan.
Biochem Biophys Res Commun. 2022 Jul 5;612:50-56. doi: 10.1016/j.bbrc.2022.04.095. Epub 2022 Apr 25.
Chondroitin sulfate (CS) and heparan sulfate (HS) are sulfated glycosaminoglycan (GAG) chains that consist of repeating disaccharide units composed of hexosamine and hexuronic acid. GAG chains exhibit diverse bioactivities in a structure-specific manner. Marine invertebrates are a rich source of highly sulfated and rare structures of GAG chains. Here, we isolated GAGs from the green-lipped mussel Perna canaliculus, an aquaculture species that is produced on a large scale. We separated GAGs based on the degree of negative charges and analyzed their disaccharide compositions. CS and HS both exhibited characteristic compositions of differently sulfated disaccharides. CS chains showed a higher degree of sulfation than HS chains and contained a high percentage of the E unit disaccharide GlcA-GalNAc(4,6-O-disulfate). Furthermore, CS chains rich in the E unit stimulated the neurite outgrowth of primary cultured neurons. The present results indicate the potential of P. canaliculus GAGs as biomaterials to study the structure-function relationships of GAGs.
硫酸软骨素（CS）和硫酸乙酰肝素（HS）是由重复二糖单位组成的硫酸化糖胺聚糖（GAG）链，这些二糖单位由己糖胺和己糖醛酸组成。GAG 链以结构特异性的方式表现出多种生物活性。海洋无脊椎动物是高度硫酸化和罕见 GAG 链结构的丰富来源。在这里，我们从大规模养殖的绿唇贻贝中分离出 GAG。我们根据负电荷程度分离 GAG，并分析其二糖组成。CS 和 HS 均表现出不同硫酸化二糖的特征组成。CS 链的硫酸化程度高于 HS 链，并且含有高比例的 E 单元二糖 GlcA-GalNAc(4,6-O- 二硫酸盐)。此外，富含 E 单元的 CS 链刺激原代培养神经元的神经突生长。这些结果表明绿唇贻贝 GAG 作为生物材料具有研究 GAG 结构-功能关系的潜力。
