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通过表达小鼠鸟氨酸脱羧酶对多胺缺陷型大肠杆菌突变体进行互补。

Complementation of a polyamine-deficient Escherichia coli mutant by expression of mouse ornithine decarboxylase.

作者信息

Macrae M, Coffino P

出版信息

Mol Cell Biol. 1987 Jan;7(1):564-7. doi: 10.1128/mcb.7.1.564-567.1987.

DOI:10.1128/mcb.7.1.564-567.1987
PMID:3550425
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC365106/
Abstract

Mouse ornithine decarboxylase (ODCase) cDNA was expressed at a high level in an Escherichia coli mutant deficient in polyamine biosynthesis. The expression of mouse ornithine decarboxylase relieved the dependence of the mutant on an exogenous source of polyamines, presumably by providing putrescine, the product of the enzyme. The effect on the enzymatic activity of deletions that removed carboxy-terminal amino acids of the protein was determined.

摘要

小鼠鸟氨酸脱羧酶（ODCase）cDNA在缺乏多胺生物合成的大肠杆菌突变体中高水平表达。小鼠鸟氨酸脱羧酶的表达缓解了该突变体对外源多胺的依赖，推测是通过提供该酶的产物腐胺来实现的。测定了去除该蛋白质羧基末端氨基酸的缺失对酶活性的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d2f/365106/26a197a1f5a2/molcellb00073-0585-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d2f/365106/26a197a1f5a2/molcellb00073-0585-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d2f/365106/26a197a1f5a2/molcellb00073-0585-a.jpg

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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Streptomycin resistance (rpsL) produces an absolute requirement for polyamines for growth of an Escherichia coli strain unable to synthesize putrescine and spermidine [delta(speA-speB) delta specC].链霉素抗性（rpsL）使得无法合成腐胺和亚精胺的大肠杆菌菌株[Δ（speA-speB）ΔspecC]生长绝对需要多胺。
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Flux control exerted by overt carnitine palmitoyltransferase over palmitoyl-CoA oxidation and ketogenesis is lower in suckling than in adult rats.与成年大鼠相比，吮乳期大鼠中肉碱棕榈酰转移酶对棕榈酰辅酶A氧化和生酮作用的通量控制较低。
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Degradation of ornithine decarboxylase: exposure of the C-terminal target by a polyamine-inducible inhibitory protein.鸟氨酸脱羧酶的降解：一种多胺诱导抑制蛋白对C末端靶标的暴露
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Multiple active conformers of mouse ornithine decarboxylase.小鼠鸟氨酸脱羧酶的多种活性构象
Biochem J. 1993 Jul 1;293 ( Pt 1)(Pt 1):289-95. doi: 10.1042/bj2930289.
8
Distinct domains of antizyme required for binding and proteolysis of ornithine decarboxylase.鸟氨酸脱羧酶结合和蛋白水解所需的抗酶不同结构域。
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Rapid and regulated degradation of ornithine decarboxylase.鸟氨酸脱羧酶的快速且受调控的降解
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