Krauss S, Lascelles C V, Zammit V A, Quant P A
Department of Biochemistry, University of Cambridge, U.K.
Biochem J. 1996 Oct 15;319 ( Pt 2)(Pt 2):427-33. doi: 10.1042/bj3190427.
We examined the potential of overt carnitine palmitoyltransferase (CPT I) to control the hepatic catabolism of palmitoyl-CoA in suckling and adult rats, using a conceptually simplified model of fatty acid oxidation and ketogenesis. By applying top-down control analysis, we quantified the control exerted by CPT I over total carbon flux from palmitoyl-CoA to ketone bodies and carbon dioxide. Our results show that in both suckling and adult rat, CPT I exerts very significant control over the pathways under investigation. However, under the sets of conditions we studied, less control is exerted by CPT I over total carbon flux in mitochondria isolated from suckling rats than in those isolated from adult rats. Furthermore the flux control coefficient of CPT I changes with malonyl-CoA concentration and ATP turnover rate.
我们使用一个概念上简化的脂肪酸氧化和生酮模型，研究了显性肉碱棕榈酰转移酶（CPT I）控制乳鼠和成年大鼠肝脏中棕榈酰辅酶A分解代谢的潜力。通过应用自上而下的控制分析，我们量化了CPT I对从棕榈酰辅酶A到酮体和二氧化碳的总碳通量的控制作用。我们的结果表明，在乳鼠和成年大鼠中，CPT I对所研究的途径都发挥着非常显著的控制作用。然而，在我们研究的条件下，与成年大鼠分离的线粒体相比，CPT I对乳鼠分离的线粒体中总碳通量的控制作用较小。此外，CPT I的通量控制系数随丙二酰辅酶A浓度和ATP周转率而变化。
