School of Environment, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; Heilongjiang Province Key Laboratory of Environmental Biotechnology, Harbin Institute of Technology, Harbin 150090, China.
School of Environment, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Sci Total Environ. 2022 Aug 25;836:155606. doi: 10.1016/j.scitotenv.2022.155606. Epub 2022 Apr 30.
Revealing the metabolic functions and the extracellular electron transfer (EET) process of psychrophilic electroactive biofilms (PEB) is important for understanding the functional adaptability of electroactive bacteria (EAB) to low temperatures. In this study, single cell sorting (SCS)-based metagenomics sequencing was used to characterize the composition and function of the PEB. Biofilm microbiome analysis showed that Geobacter, with a relative abundance of 62.64%, dominated the PEB enriched in the bioelectrochemical system (BES) at 4 °C. Both the metagenome (MAG) and single-cell metagenome (SCM) revealed that there were no obvious metabolic differences between the original biofilm and the sorted single cells. The presence of genes associated with type IV pilus, c-type cytochromes, and riboflavin indicated that the EET potential was maintained in the PEB at low temperatures. These results suggested that SCM provides an alternative approach to reconstruct the metabolic functions of uncultured and slow-growing EAB.
揭示嗜冷电活性生物膜（PEB）的代谢功能和细胞外电子转移（EET）过程对于理解电活性细菌（EAB）对低温的功能适应性非常重要。在本研究中，基于单细胞分选（SCS）的宏基因组测序用于表征 PEB 的组成和功能。生物膜微生物组分析表明，在 4°C 下富集的生物电化学系统（BES）中，相对丰度为 62.64%的 Geobacter 主导了 PEB。宏基因组（MAG）和单细胞宏基因组（SCM）都表明，原始生物膜和分选的单个细胞之间没有明显的代谢差异。与 IV 型菌毛、c 型细胞色素和核黄素相关的基因的存在表明，在低温下，PEB 中的 EET 潜力得以维持。这些结果表明，SCM 为重建未培养和生长缓慢的 EAB 的代谢功能提供了一种替代方法。
