Laboratory of Photodynamic Inactivation of Microorganisms, Department of Biosciences and Medical Biology, Paris Lodron University Salzburg, Salzburg, Austria.
Methods Mol Biol. 2022;2451:691-699. doi: 10.1007/978-1-0716-2099-1_36.
Unsafe food causes more than 200 diseases and therefore poses a threat to the health of millions of people worldwide. Children under 5 years of age carry about 40% of the foodborne disease burden. With a rapidly growing world population, the supply of nutritious, safe, and healthy food represents a high challenge for the coming centuries. Photodynamic decontamination of food (PDc) is based on the photosensitizer (PS)-mediated and light-induced overproduction of reactive oxygen species, which kill microorganisms irrespective of their resistance to conventional treatment. Several natural substances approved as food additives such as curcumin or chlorophyllin are photoactive. Thus, PDc based on these compounds is a promising approach to improve food safety.In this chapter, two experimental protocols to investigate the antimicrobial efficacy of PDc on flat objects like lettuce or slices of cucumber or round objects like mung beans in situ are described in detail, which allow for quantitative analysis of the decontamination effect. Both methods are also applicable for other radiation-based decontamination, such as UV- or γ-treatment of food.
不安全的食物会导致 200 多种疾病，从而威胁到全世界数百万人的健康。5 岁以下儿童承担着大约 40%的食源性疾病负担。随着世界人口的快速增长，提供营养、安全和健康的食物是未来几个世纪面临的巨大挑战。食品光动力消毒（PDc）基于光敏剂（PS）介导和光照诱导的活性氧过度产生，无论微生物对常规处理的抗性如何，都会杀死它们。几种被批准为食品添加剂的天然物质，如姜黄素或叶绿酸，具有光活性。因此，基于这些化合物的 PDc 是提高食品安全的一种有前途的方法。在这一章中，详细描述了两种实验方案，用于研究 PDc 对生菜或黄瓜片等扁平物体或绿豆等圆形物体的原位抗菌功效，这两种方法都允许对消毒效果进行定量分析。这两种方法也适用于其他基于辐射的消毒，如食品的 UV 或γ处理。
